Abstract: The preparation and use of an anti-F SHR antibody and an antibody-drug conjugate thereof. Specifically, an antibody or an antigen-binding fragment thereof is disclosed. The antibody or the antigen-binding fragment thereof contains a variable region and specifically binds to human follicle-stimulating hormone receptor FSEIR, and contains one, two, three, four, five or six CDRs with the following amino acid sequences shown in SEQ ID NOs: 6, 7, 8, 10, 11 and 12.