Abstract: The present invention relates to cell and tissue culture. In particular, the present invention provides a method for preparing an organotypic culture using dissociated cells or microexplants obtained from an animal organ. The method for preparing an organotypic culture comprises culturing cells from an organ on a surface characterized in that the cells are compacted. The invention further relates to a high-throughput method for the preparation of a collection of organotypic cultures. The invention further relates to a device for carrying out a method of organotypic culture according to the invention.

Abstract: The present invention relates to the field of cell and tissue culture. In particular, the invention provides a novel device for organotypic culture. The device comprises a medium conduit having one open end and one end closed by a porous membrane fused across it; and a frame holding the medium conduit in a substantially vertical orientation; wherein the medium conduit is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane and thus supply nutrients to cells that may be grown on the porous membrane. The device may further comprise a cell suspension conduit having one end open and one end closed by the surface of said porous membrane contralateral to the surface of said porous membrane sealed to said medium conduit; wherein said frame holds the medium conduit and the cell suspension conduit in a substantially vertical orientation.

Abstract: The present invention relates to the field of cell and tissue culture. In particular, the invention provides a novel device for organotypic culture. The device comprises a medium conduit having one open end and one end closed by a porous membrane fused across it; and a frame holding the medium conduit in a substantially vertical orientation; wherein the medium conduit is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane and thus supply nutrients to cells that may be grown on the porous membrane. The device may further comprise a cell suspension conduit having one end open and one end closed by the surface of said porous membrane contralateral to the surface of said porous membrane sealed to said medium conduit; wherein said frame holds the medium conduit and the cell suspension conduit in a substantially vertical orientation.