Abstract: The invention is directed to an arrangement for light sheet microscopy. An arrangement of this type comprises illumination optics with an illumination objective (5) for illuminating a sample (3) with a light sheet via an illumination beam path, which sample (3) is located on a sample carrier in a medium (2), wherein the optical axis (6) of the illumination objective (5) and the light sheet lie in a plane which forms an illumination angle (?) not equal to zero with the normal lines of a plane reference surface (4) with respect to which the sample carrier is aligned. The arrangement further comprises detection optics with a detection objective (7) in a detection beam path, the optical axis (8) of the detection objective (7) forming a detection angle (?) not equal to zero with the normal lines of the reference surface (4).