Abstract: In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

Abstract: The present invention relates to yeast strains of the species Saccharomyces pastorianus with the useful characteristic of producing low levels of diacetyl during fermentation. Also provided are methods of producing a malt and/or cereal based beverage with these strains, as well as beverages produced by this method.

Abstract: The invention relates to yeast strains with useful characteristics, including not being capable of producing phenolic off-flavors and/or not capable of utilizing maltose or which has limited ability to utilize maltose. Also provided is methods of producing cereal based beverages without phenolic off-flavors and/or a low alcohol or a non-alcoholic malt and/or cereal based beverage, as well as beverages produced by these methods.

Abstract: The present invention relates to barley plant or a part thereof, wherein the kernels of said barley plant have a reduced (1,3;1,4)-?-glucan content. The barley plant may carry a mutation in the CslF6 gene, wherein said mutated CslF6 gene encodes a mutant CslF6 polypeptide.

Abstract: The present invention provides barley plants, or parts thereof, having a high limit dextrinase activity. In particular, barley plants carrying a mutation in HvLDI gene are provided. Furthermore, plant products prepared from said barley plants, or parts thereof, are described as well as methods of producing the same.

Abstract: The present invention provides methods of preparing plants, with specific predetermined mutation(s) in one or more NOI(s). The specific predetermined mutation(s) preferably may result in the identification of plants having desired traits.

Abstract: The present invention relates to the field of beverages or beverage bases, in particular to the field of health beverages. The beverage or beverage base is produced by preparing an aqueous extract of unmalted cereal grains. Fermenting the aqueous extract by cold contact fermentation and/or with inactivated yeast to obtain a fermented aqueous extract. The aqueous extract or fermented aqueous extract is then mixed with a juice, to obtain an alcohol free or low alcohol beverage or beverage base with desirable ingredients.

Abstract: In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms.

Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off-flavors, even after prolonged storage of the beverage.

Abstract: A method of coating hydroxylated surfaces by gas phase grafting is described. Especially acyl groups, silyl groups and/or alkyl groups are located on the surface of materials by gas phase grafting. The grafting method is a dry process. The material to coat can be organic or inorganic materials. The produced surface coated material are strong and durable and material normally not water-proof can be water-proof due to the surface treating. Examples of items produced from surface treated material may be water-proof cardboard boxes, other containers, furniture, interior for cars and boats. Items produced from organic materials such as from plant parts are biodegradable.

Abstract: A method and a system for process parameter control of a liquid composition in a reverse electro-enhanced dialysis (REED) system comprising at least two Reverse Electro-Enhanced Dialysis (REED) membrane stacks, wherein the direction of the electric field within any one membrane stack is reversed at asynchronical intervals of time relative to the current reversals for any other membrane stack.

Abstract: A process for simultaneously controlling the pH and level of target ions of a liquid composition, more particularly the use of Reverse Electro-Enhanced Dialysis (REED) for extraction of low-molecular, charged species generated in a reactor. Even more particularly, the invention relates to a process for simultaneous pH control and control of inhibitors in an ongoing fermentation or catalytical system, where the pH is controlled by at least one primary Reverse Electro-Enhanced Dialysis (REED) stack and the level of target ions by at least one secondary REED stack, where the primary REED is one of a Cation REED (CX-REED) or an Anion REED (AX-REED) and the secondary REED is the other of a CX-REED and an AX-REED.

Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.

Abstract: A method and a system for process parameter control of a liquid composition in a reverse electro-enhanced dialysis (REED) system comprising at least two Reverse Electro-Enhanced Dialysis (REED) membrane stacks, wherein the direction of the electric field within any one membrane stack is reversed at asynchronical intervals of time relative to the current reversals for any other membrane stack.

Abstract: The invention relates to a polymer material with several advantageous properties. The polymer material has a low degree of swelling in water, it is biodegradable, it has extremely low permeability to gasses such as CO2 and it has a high strength. The polymer material is useful for a number of purposes, such as for preparing containers for food or beverages. The polymer material is prepared from a prepolymerization mixture comprising grafted plant derived material and monomers and/or cross-linkers.

Abstract: A method of coating hydroxylated surfaces by gas phase grafting is described. Especially acyl groups, silyl groups and/or alkyl groups are located on the surface of materials by gas phase grafting. The grafting method is a dry process. The material to coat can be organic or inorganic materials. The produced surface coated material are strong and durable and material normally not water-proof can be water-proof due to the surface treating. Examples of items produced from surface treated material may be water-proof cardboard boxes, other containers, furniture, interior for cars and boats. Items produced from organic materials such as from plant parts are biodegradable.

Abstract: The present invention relates to methods for identifying compounds capable of modulating a cellular response. The methods involve attaching living cells to solid supports comprising a library of test compounds. Test compounds modulating a cellular response, for example via a cell surface molecule may be identified by selecting solid supports comprising cells, wherein the cellular response of interest has been modulated. The cellular response may for example be changes in signal transduction pathways modulated by a cell surface molecule.

Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.

Abstract: According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.

Abstract: The present invention is related to methods for the identification of spatially encoded beaded or granulated matrices comprising a plurality of immobilised particles. The identification is based on a distance matrix determination or based on a set of geometrical figures, such a triangles, on the basis of which individual matrices can be determined.