Abstract: A method of target DNA genome analysis is provided. The method comprises the steps of: —obtaining non-overlapping segments of target DNA stretches with segment boundaries defined by the presence of particular restriction enzyme recognition sites, whereby the assembly of said non-overlapping segments compose a reduced representation library of said target DNA genome; —obtaining for said segments, raw metrics from a sequencing process applied on said reduced representation library; —clustering non-overlapping, nearby segments with similar raw metrics to provide master segments; —providing metrics describing the master segments, —making a final discrete DNA call based on the master segments and its metrics.