Abstract: The present invention relates to a culture vessel for three-dimensional cell cultivation and a three-dimensional cell co-cultivation method using the same. The culture vessel comprises a well formed by a column positioned thereon and at least one support protruding from the column within the well. In contrast to conventional techniques, the present invention allows cells to be cultured at a position spaced from a culture vessel, thus enjoying the advantage of smoothly supplying oxygen necessary for three-dimensional cell culture structures.

Abstract: A three-dimensional cell culture method on the basis of a biodegradable synthetic hydrogel is provided. The method may include culturing cells in a cell culture dish in a non-contact manner using a porous membrane and a biodegradable synthetic hydrogel in the culture of stem cells and primary cells.

Abstract: The present invention relates to a method for inducing osteogenic differentiation of mesenchymal stem cells and, more particularly, to a short-time osteogenic differentiation method of culturing cells using a porous membrane and a biodegradable synthetic biogel, whereby the cells do not contact a cell culture container. The present invention can significantly shorten the induction period of osteogenic differentiation, compared to the conventional osteogenic differentiation method, and has an effect of the cells being easily separable after differentiation as well.

Abstract: The present invention relates to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, and use thereof, more particularly, to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, in which the albumin, lipids, and plant protein hydrolysates are present in sufficient quantities to reduce cell death caused by freezing while maintaining animal-free and xeno-free conditions during long-term preservation of stem cells or primary cells; and to a method for cryopreservation of stem cells or primary cells using the composition.