Abstract: Compositions and methods are described for enhancing the immunohistochemical staining of tissue samples. Compositions include a single solution, which includes at least one surfactant, adapted to remove an embedding medium of a tissue sample, rehydrate the tissue sample, and enhance the immunohistochemical staining of the tissue in relation to immunohistochemical staining of unreacted tissue. Methods include heating the tissue sample with the composition to enhance the immunohistochemical staining of the tissue.

Abstract: A system and method for applying one or more chemicals to a tissue sample is provided. The system preferably includes a cassette for housing a slide device, a film, and an injection system. The slide device preferably includes a specimen slide for containing the tissue sample and a cover plate connected to the specimen slide. The film is preferably moveable through the cassette along guide rollers and preferably contains a plurality of containers containing one or more chemicals. The injection system may include a piston for displacing the chemicals from the containers through an injection port to the tissue sample. The cassette may be placed on a cassette driver that contains a motor-driven shaft for driving the piston and moving the film.