Abstract: The present application discloses a liquid composition for cryopreservation of a biological material comprising a polar aprotic solvent, a monohydric or polyhydric alcohol, an unbranched polysaccharide, a branched polysaccharide; and polyvinyl alcohol. Further provided are uses of said liquid composition for cryopreservation of abiological material and methods for preserving a biological material using said liquid composition.