Abstract: A cell and tissue culture unit comprises a base (1) equipped with access means (7,8) for being connected to a culture medium feed device and delimiting at least side-walls (5) of a culture chamber (6) and at least side-walls of at least a well (3-i ) communicating through at least one of its upper and lower portions with chamber (6), as well as removable closing means (10,15-i,17i) able to provide access to chamber (6) and to well (3-i).

Abstract: A cell and tissue culture device comprises several culture wells (18-i) accommodating cells and tissues to be grown, of first (2) and second (25) tanks, and connecting means (20,23,24) coupled to wells and tanks so as to enable a culture fluid to flow from one tank to another via the wells. Each tank (20,25) accommodates at least a flexible pocket (6,7;27,29), at least one of which is able to receive the culture fluid. The device further comprises at least a controller module (50,150) providing first and/or second sequences of external pressures to be applied, on the pockets of first and second tanks, respectively, and pressurization means (41,46-49) configured for applying to the pockets, pressures defined by said first and second sequences provided by the controller module.