Abstract: Method for preserving tissue including immersing the tissue in a solution having a cryoprotectant concentration of at least 75% by weight, a cooling step where the tissue is cooled to a temperature between the glass transition temperature of the solution having a cryoprotectant concentration of at least 75% by weight and ?20° C., a storage step where the tissue is stored at a temperature between the glass transition temperature of the solution and ?20° C., a rewarming step, where the tissue is warmed, and a washing step.
Type:
Grant
Filed:
February 23, 2010
Date of Patent:
May 14, 2013
Assignees:
Lifeline Scientific, Inc., Cell & Tissue Systems, Inc.
Abstract: Methods for ex vivo perfusion of organs (and/or tissues) with a perfusate designed to condition the organ with the desired effect being that upon transplant, said organ, having been administered said perfusate, is less likely to experience delayed graft function, deleterious effects of ischemia/reperfusion injury, including inflammatory reactions, and/or other detrimental responses that can injure the organ or recipient including precipitating or enhancing an immunological reaction from the recipient with the potential of compromising the graft's and/or recipients short teen and/or long term health and proper functionality while monitoring, sustaining and/or restoring the viability of the organ and preserving the organ for storage and/or transport.
Type:
Application
Filed:
June 7, 2012
Publication date:
December 13, 2012
Applicants:
CELL & TISSUE SYSTEMS, INC., LIFELINE SCIENTIFIC, INC.
Inventors:
David KRAVITZ, Kelvin G. M. BROCKBANK, Lia H. CAMPBELL
Abstract: Methods of preparing pancreatic islets that may comprise pancreatites are provided. The methods may involve subjecting a pancreas and/or pieces thereof to disruption, such as, for example, an enzyme digest, and seeding the recovered cellular product comprising islets in a culture medium comprising at least a detectable amount of endocrine tissue and/or exocrine tissue.
Abstract: Methods of isolating cellular products, such as pancreatic islets, may be used in diabetes research and therapeutic transplantation. The methods may involve providing a donor tissue having desired cells and undesired cells, perfusing the donor tissue with a perfusion solution, developing edema during perfusion of the donor tissue to form a swelled tissue, and separating the desired cells from undesired cellular material to obtain a cellular product. The methods may also include disrupting the tissue, and separating the desired cells from undesired cellular material to obtain the cellular product. The methods may result in an increased yield of cellular product that retains sufficient functional integrity to be useful as a transplantation resource.
Type:
Application
Filed:
July 15, 2011
Publication date:
January 19, 2012
Applicants:
CELL & TISSUE SYSTEMS, INC., LIFELINE SCIENTIFIC, INC.
Inventors:
Michael J. TAYLOR, Simona C. BAICU, David KRAVITZ
Abstract: Method for preserving tissue including immersing the tissue in a solution having a cryoprotectant concentration of at least 75% by weight, a cooling step where the tissue is cooled to a temperature between the glass transition temperature of the solution having a cryoprotectant concentration of at least 75% by weight and ?20° C., a storage step where the tissue is stored at a temperature between the glass transition temperature of the solution and ?20° C., a rewarming step, where the tissue is warmed, and a washing step.
Type:
Application
Filed:
February 23, 2010
Publication date:
August 26, 2010
Applicants:
CELL & TISSUE SYSTEMS, INC., LIFELINE SCIENTIFIC INC.