Abstract: Methods of expanding and selecting disease associated T-cells, continuous T-cell lines as well as T-cell lines obtainable by these methods are disclosed. Furthermore, pharmaceutical compositions and vaccines comprising activated disease associated T-cell are disclosed. The uses of the T-cell and T-cell lines are numerous and include methods of diagnosis, methods for the treatment, alleviation or prevention of diseases associated with activation of T-cells, methods of testing the effect of medicaments against T-cell associated diseases, methods of detecting T-cell growth factors, methods of monitoring the response to treatment, alleviation or prevention of diseases associated with activation of T-cells, and methods of identifying disease associated antigens.