Abstract: A three-dimensional (3D) bioprinting method and system are disclosed. The method includes disposing/immersing a printing platform or surface into a first bioink, such as a bioink resin, curing one or more layer of the first bioink resin onto the printing platform or surface, and removing the printing platform or surface from the first bioink resin. The process is repeated with a second bioink resin such that the second bioink resin is cured on top of the one or more layer of first bioink resin, and can be further repeated with a third or even fourth bioink resin. By varying constituents of one or more or each bioink resin (such as living cell type or polymer), complex, multilayered tissues can be engineered. A system capable of performing the method is also disclosed.

Abstract: A perfusion device for printing a 3D construct with internal channels is disclosed. The device comprises a housing with an internal chamber, the chamber configured to hold the construct, and having a bottom plate configured to support the construct, the chamber comprising at least one inlet or outlet configured to introduce a liquid from outside the chamber into the chamber or vice versa. The at least one inlet or outlet comprises an connector adapted to connect or align the one or more internal channels within the 3D construct to the inlet or outlet when the 3D construct is present in the chamber. Further a method for printing a 3D construct with at least one internal channel is disclosed.

Abstract: The present invention relates to a double network bioink that is customizable and tailorable in terms of mechanical characteristics, diffusivity, and biological functionality that is printable at room temperature and cytocompatible. Double network bioinks of the present invention can be utilized as a standard for comparison and evaluation of bioinks and during calibration and/or are suited for the education field as a “blank slate” bioink that can be utilized to convey different scientific concepts. Specific bioinks included comprise: one or more biocompatible or non-biocompatible thickener; one or more polyethylene glycol based crosslinkable network; one or more photoinitiator; and/or optionally, one or more additives to impart desired and/or different characteristics to the bioink. In embodiments the thickener and polyethylene glycol based crosslinkable network form a structure comprising two interpenetrating networks.

Abstract: The present disclosure relates to a print bed (1) for regulating a temperature of the print bed (1). The print bed (1) comprises at least one Peltier element (2), each Peltier element having opposite first and a second surfaces (3a, 3b). The print bed (1) further comprises at least one heatsink (4). The at least one Peltier element (2) is arranged to have each respective first surface (3a) facing a print surface (5) of the print bed (1). The at least one heatsink (4) is thermally connected to the Peltier element (2) and arranged to transfer heat generated by the at least one Peltier element (2) and dissipate the transferred heat away from the at least one Peltier element (2). The present disclosure further relates to corresponding 3D-printers, methods, computer programs and modules.

Abstract: The present invention relates to biomaterial in the form of dispersion of cellulose nanofibrils with extraordinary shear thinning properties which can be converted into desire 3D shape using 3D Bioprinting technology. In this invention cellulose nanofibril dispersion, is processed through different mechanical, enzymatic and chemical steps to yield dispersion with desired morphological and rheological properties to be used as bioink in 3D Bioprinter. The processes are followed by purification, adjusting of osmolarity of the material and sterilization to yield biomaterial which has cytocompatibility and can be combined with living cells. Cellulose nanofibrils can be produced by microbial process but can also be isolated from plant secondary or primary cell wall, animals such as tunicates, algae and fungi. The present invention describes applications of this novel cellulose nanofibrillar bioink for 3D Bioprinting of tissue and organs with desired architecture.

Abstract: The present invention relates to biomaterial in the form of dispersion of cellulose nanofibrils with extraordinary shear thinning properties which can be converted into desire 3D shape using 3D Bioprinting technology. In this invention cellulose nanofibril dispersion, is processed through different mechanical, enzymatic and chemical steps to yield dispersion with desired morphological and rheological properties to be used as bioink in 3D Bioprinter. The processes are followed by purification, adjusting of osmolarity of the material and sterilization to yield biomaterial which has cytocompatibility and can be combined with living cells. Cellulose nanofibrils can be produced by microbial process but can also be isolated from plant secondary or primary cell wall, animals such as tunicates, algae and fungi. The present invention describes applications of this novel cellulose nanofibrillar bioink for 3D Bioprinting of tissue and organs with desired architecture.