Abstract: Linker compounds for formation of stably-linked conjugates are disclosed. Such linker compounds are aminooxy-containing linker compounds useful in forming conjugates having stable oxime linkages. The stably-linked conjugates have utility in a variety of immunodiagnostic and separation techniques.

Abstract: Linker compounds for formation of stably-linked conjugates are disclosed. Such linker compounds are semicarbazide-containing linker compounds useful in forming conjugates having stable semicarbazone linkages. The stably-linked conjugates have utility in a variety of immunodiagnostic and separation techniques.

Abstract: A particle separator is provided for collection and manipulation of target particles, e.g., target cells, in a closed sterile field condition. In one embodiment, closed sterile field conditions are maintained from separation through concentration and/or cryo treatment steps and/or transfusion. Preservation of closed sterile field condition are accommodated by using the same integrally coupled rigid-walled vessel for collection and concentration and transfer via integrally coupled conduits to a vessel for cryopreservation and/or transfusion.In one embodiment, a plurality of valves are responsive to a data processor for controlling the path of fluid flow through the particle separator. A plurality of sensors are provided for providing sensor signals indicative of fluid flowing through the cell separator. A peristaltic pump is responsive to the microprocessor assembly for controlling the speed and direction of fluid flow through the system.

Abstract: A method is provided for removing a second ligand from a particle surface without substantially affecting the particle surface, comprising the step of exposing the particle to a first ligand immobilized onto a support, wherein the particle is exposed under conditions and for a residence time sufficient to allow the second ligand to desorb from the particle surface, and wherein the first ligand has an affinity for the second ligand that is at least two orders of magnitude greater than the affinity of the second ligand for the particle surface, such that the second ligand is removed from the particle surface without substantially affecting the particle surface.

Abstract: A cell separator is provided that includes apparatus for automatically controlling the cell separation process. Particularly, a plurality of valves are responsive to a data processor assembly for controlling the path of fluid flow through the cell separator. A plurality of sensors are provided for providing sensor signals indicative of the density of fluid flowing through the cell separator. The microprocessor is responsive to the sensor signals for controlling the flow and operation of the cell separator. A parastaltic pump is responsive to the microprocessor assembly for controlling the speed and direction of fluid flow through the system. A stirplate assembly is responsive to a drive signal from the data processor assembly for controllably agitating the contents of a column. The data processor assembly is further responsive to the sensor input to provide the valve control signals and pump control signal to control the concentration of selected cells that are collected.