Abstract: A flexible bioreactor intended for culturing cells in a liquid medium, the bioreactor comprising a flexible wall forming a fluid impermeable bottom wall of the bioreactor when in use, wherein flexible parts of said flexible bottom wall are interrupted by at least one rigid element of said flexible bottom wall, the rigid element and the flexible parts being connected fluid tightly, and wherein the rigid element comprises measuring means allowing for non-invasive monitoring of cellular activity in said bioreactor when in use.

Abstract: A method for cultivating cells utilizing wave motion comprises the steps of: providing a container; introducing a gas containing oxygen, a liquid medium and a cell culture into the container; moving the container such that the container swivels with respect to a substantially horizontal pivot axis to thereby induce a wave motion to the liquid medium in the container, which wave motion contributes to the necessary oxygen transfer and mixing required for cell growth. During said swivelling of the container said pivot axis follows a cyclical closed-loop path. Also provided is an apparatus for performing said method, and an assembly for said method. Further part of the invention are disposable bags for cell or bacterial culture of which the volume can be changed in situ by opening or closing compartments of the bag and/or bags with essentially straight corners.