Abstract: The inventive method for automatically analysing a cellular sample consists (a) in colouring the cell nucleuses of a sample, (b) inobtaining at least one digital picture of the sample, and (c) in digitally analysing said image and is characterised in that the cell nucleus colouring stage (2) is embodied in the form of a DNA-non-specific colouring. In order to use the inventive method, said invention also relates to a method for selecting a culture medium, a method for measuring substance toxicity and to a method for measuring the cytopathological characteristics of a virus. A method for selecting pharmacological substances for treating excess weight and obesity diseases and a method for selecting substances for treating osteoporosis are also disclosed.
Abstract: The invention concerns a composition of culture medium of progenitor/stem cells derived from muscular tissues containing serum and/or serum fraction of human origin and/or of animal origin of insulin or a derivative thereof, and one or several compound(s) selected among the class of antioxidants and/or of vitamins. The invention also concerns a method for culturing progenitor/stem cells, a method for producing myoblasts capable of being used as cellular/genetic therapy product. The invention aims at optimizing the production of myoblasts from progenitor/stem cells.
Abstract: The invention relates to tissue regeneration and repair by cell transplant. More particularly, the invention concerns a method for preparing adult stem cells which preserves the diversity and plasticity of said cells. The invention also concerns the use of said stem cells obtained by the inventive extraction method for therapeutic uses, including gene and cell therapy. The inventive preparation method essentially consists in: cell extraction, mechanical dissociation, enzymatic dissociation, maintaining the cells obtained in a specific culture medium enabling preservation of diversity and plasticity, said method excluding cell culture.
Type:
Application
Filed:
June 27, 2003
Publication date:
June 8, 2006
Applicants:
Institut Pasteur, Celogos, Fondation De L'avenir, Centre National De La Recherche Scientifique
Inventors:
Didier Montarras, Nicolas Borenstein, Christian Pinset