Abstract: The present invention belongs to animal experiments field, disclosed a single factor modeling-constraint method and device for mice chronic fatigue syndrome, the modeling-constraint method is specifically using mineral water bottle to restraint mice; the mineral water bottle is divided into upper, middle and lower parts; the body of mineral water bottle is uniformly drilled for mice to breathe; the inner of the mineral water bottle is blocked by a cardboard to reduce the move space of mice; cover the mice through the divided bottle; one end of the opening is tightly attach to the wall, the other end is tightly attach to the mice feeding box, and keeping 30 minutes to restraint. The present invention utilizes mineral water bottle to make three simple restraint tube, not only saves the costs of experiment, but also turns waste into treasure, which is a simple operation, and increases the possibility of the experiment.

Abstract: A laboratory zebrafish autism behavioral model apparatus is provided. The laboratory zebrafish autism behavioral model apparatus comprises a model body, wherein the model body is divided into a plurality of experimental units, and each of the experimental units has a cuboid structure with completely identical shapes; a free swimming area, a stimulus area, and a normal non-stimulus area are formed on each of the experimental units; the stimulus area and the normal non-stimulus area are located on two sides of the free swimming area, respectively, and the free swimming area, the stimulus area, and the normal non-stimulus area are interconnected via mesh openings. The zebrafish autism behavioral model apparatus facilitates further exploration of autism etiology, effectively resolving interference between the stimulus area and the zebrafish's innate thigmotactic behavior, reducing experimental errors, saving time, and minimizing instrument wear and costs.

Abstract: The subject invention pertains to isolated influenza virus that is capable of infecting canids and causing respiratory disease in the canid. The subject invention also pertains to compositions and methods for inducing an immune response against an influenza virus of the present invention. The subject invention also pertains to compositions and methods for identifying a virus of the invention and diagnosing infection of an animal with a virus of the invention.

Abstract: The present application relates to one or more fusion proteins, comprising a structural domain of SARS-CoV-2 S(Spike) protein. The present application also relates to an immunogenic composition comprising the fusion protein and an application thereof.

Abstract: The present application proposes a method for detecting an okadaic acid based on a near-infrared photoelectric composite material, which includes the following steps: synthesizing NaYF4: Yb, Tm up-conversion nanoparticles (UCNPs) and a semiconductor material flower-like tungsten oxide (WO3) by a simple high-temperature solvothermal method; coupling the UCNPs with an okadaic acid monoclonal antibody through a classic amidation reaction to construct a competitive near-infrared photoelectrochemical immunosorbent assay (cNIR-PECIA) for okadaic acid detection. In addition, the present application employs a screen-printed carbon electrode (SPE) as the working electrode, and thus only requires a small amount of electrolytes, which is low-cost and maintenance-free.

Abstract: Medical protective clothing, including a protective clothing body. The protective clothing body includes a hood part, a neck part, a top, pants, shoe cover parts, and sleeves, and is formed by sewing the hood part, the neck part, the top, the pants, the shoe cover parts and the sleeves in sequence. The hood part includes an air exchange assembly. The sidewall of the top is separated from the back of the medical personnel by means of airbag ribs, thus preventing the sweaty back from being in contact with the sidewall of the top to make the people feel uncomfortable when the protective clothing is worn for a long time in summer.

Abstract: Provided is a diagnostic system for identifying target microorganisms and/or resistance genes in a sample, including a cell lysis unit, a target nucleic acid enriching unit, a sequencing unit, and a sequence analyzing unit, wherein the cell lysis unit is configured to lyse non-target cells in the sample, the target nucleic acid enriching unit equipped with an immobilized adsorption device is configured to deplete nucleic acids of the non-target cells and to enrich nucleic acids of the target microorganisms, and the sequencing unit and the sequence analyzing unit are configured to produce identification results of the microbial species and/or resistance genes from the sequences of the enriched nucleic acids. Also provided is a method for enriching target nucleic acids in a sample and a method for identifying target microorganisms and/or resistance genes by sequencing the enriched nucleic acids of the target microorganisms.

Abstract: The present invention provides a method of detecting Staphylococcus aureus in a subject, by contacting a sample obtained from the subject with at least one detectably labeled probe of the invention or detecting in the sample identity to a sequence of the invention. The invention is also directed to kits, microarrays and detectable Staphylococcus aureus polynucleotide probes useful in detecting the presence of Staphylococcus aureus.

Abstract: Methods of producing a pathogen with reduced replicative fitness are disclosed, as are attenuated pathogens produced using the methods. In particular examples, the method includes deoptimizing one or more codons in a coding sequence, thereby reducing the replicative fitness of the pathogen. Methods of using the attenuated pathogens as immunogenic compositions are also disclosed.

Abstract: The present application proposes a method for detecting an okadaic acid based on a near-infrared photoelectric composite material, which includes the following steps: synthesizing NaYF4: Yb, Tm up-conversion nanoparticles (UCNPs) and a semiconductor material flower-like tungsten oxide (WO3) by a simple high-temperature solvothermal method; coupling the UCNPs with an okadaic acid monoclonal antibody through a classic amidation reaction to construct a competitive near-infrared photoelectrochemical immunosorbent assay (cNIR-PECIA) for okadaic acid detection. In addition, the present application employs a screen-printed carbon electrode (SPE) as the working electrode, and thus only requires a small amount of electrolytes, which is low-cost and maintenance-free.

Abstract: The present application provides a method for preparing a probiotic-loaded microcapsule including: (1) dissolving sodium alginate in a solvent to obtain a sodium alginate solution; (2) evenly mixing a probiotic with the sodium alginate solution obtained in step (1) to obtain a probiotic suspension; and (3) spraying the probiotic suspension obtained in step (2) into a salt solution, stirring and curing, to obtain the probiotic-loaded microcapsule. The microcapsule is prepared by a spraying method in conjunction with an ion curing method by using sodium alginate as the wall material of the microcapsule, so that the prepared microcapsule has a good spherical shape, a small particle size, good dispersibility and high pH sensitivity, forms a denser surface under an acidic condition to effectively protect probiotics, and can meet requirements for subsequent in vivo animal evaluations.

Abstract: The present invention relates to a recombinant antigen derived from Zika virus E protein and use thereof. Specifically, the present invention provides a polynucleotide encoding Zika virus E protein domain III alone or repeatedly three times, a recombinant plasmid vector comprising the polynucleotide, and a DNA vaccine composition that may induce an immune response to Zika virus by expressing a Zika virus antigen protein effectively. In addition, the present invention provides a neutralizing antibody against Zika virus obtained using the polynucleotide and a method for preparing the neutralizing antibody.

Abstract: The subject invention pertains to isolated influenza virus that is capable of infecting canids and causing respiratory disease in the canid. The subject invention also pertains to compositions and methods for inducing an immune response against an influenza virus of the present invention. The subject invention also pertains to compositions and methods for identifying a virus of the invention and diagnosing infection of an animal with a virus of the invention.

Abstract: A dispersive solid-phase extraction material, a preparation method therefor and an application thereof. The method comprises: weighing humic acid and washing it using water under ultrasonic bath, centrifuging to obtain a solid precipitate; resuspending the solid precipitate in acetone, heating and evaporating until the acetone has evaporated completely to then obtain a residue; taking and placing the residue in a Soxhlet extractor, and adding a cleaning solution; cleaning by heating and refluxing until the refluxed liquid is clear and colorless, and then heating is stopped; taking out the cleaned material; drying the material at 100° C. for 1.5-2.5 h, cooling for 0.5-1 h, and sieving through a 120 mesh sieve; collecting the material under the sieve to obtain the dispersive solid-phase extraction material. The dispersive solid-phase extraction material can simultaneously extract and purify aflatoxins in edible oils for their detection.

Abstract: Broadly neutralizing antibodies against HIV-1 which specifically bind to gp120 of HIV-1, a method of preparing such antibodies and the use thereof are provided.

Abstract: Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase, protease, or integrase of HIV as disclosed herein. Thus, provided in an oligonucleotide comprising any one of the nucleotide sequences set forth in SEQ ID NOS: 1-89, 96-122, and 124-141. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS: 1-89, 96-122, and 124-141. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCT reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV simultaneously or sequentially. Mutations in the reverse transcriptase, protease, or integrase of HIV can be detected using the methods described herein.

Abstract: Methods, kits and devices for detecting antineoplastic drug contamination of a surface are provided according to aspects of the present invention. According to aspects of the invention, methods for detecting antineoplastic drug contamination of a surface include providing a wetting solution compatible with the antineoplastic drug and formulated to promote release of the drug from the surface to be assayed; providing a solid matrix for reversible absorption of the antineoplastic drug; contacting the solid matrix with the wetting solution, generating an assay matrix; contacting the assay matrix and the surface, generating a surface sample; contacting the surface sample with a volume of wetting solution, generating a fluid test sample; and quantifying the antineoplastic drug in the fluid test sample by lateral flow assay to produce an assay result, thereby detecting antineoplastic drug contamination of the surface.

Abstract: The present invention relates to a recombinant antigen derived from Zika virus E protein and use thereof. Specifically, the present invention provides a polynucleotide encoding Zika virus E protein domain III alone or repeatedly three times, a recombinant plasmid vector comprising the polynucleotide, and a DNA vaccine composition that may induce an immune response to Zika virus by expressing a Zika virus antigen protein effectively. In addition, the present invention provides a neutralizing antibody against Zika virus obtained using the polynucleotide and a method for preparing the neutralizing antibody.

Abstract: Methods of producing a pathogen with reduced replicative fitness are disclosed, as are attenuated pathogens produced using the methods. In particular examples, the method includes deoptimizing one or more codons in a coding sequence, thereby reducing the replicative fitness of the pathogen. Methods of using the attenuated pathogens as immunogenic compositions are also disclosed.

Abstract: Provided are antigenic polypeptides of HIV envelope glycoproteins which are constructed based on amino acid mutation of attenuated live vaccine of Equine Infectious Anemia Virus, DNA constructions and recombinant virus vectors comprising polynucleotides encoding said polypeptides, antibodies against said polypeptides as well as uses thereof in preventing and treating HIV infection. Said antigenic polypeptides and vaccines can induce high titer neutralization antibodies against HIV in organism.