Patents Assigned to CENTER FOR QUANTITATIVE CYTOMETRY
  • Patent number: 10670512
    Abstract: The Intrinsic Hyper-Spectral Flow Cytometer (IHSFC) and its associated methodology, improves current flow cytometry by eliminating the need of associated hardware-based elements currently used for spectral data detection. The (IHSFC), rather than using narrow band lasers to excite or interrogate the analytes, the flow stream is excited by a wide wavelength range beam. The raw data generated by the (IHSFC) are as follows; forward light scatter, right angle light scatter, coherent spectral data and non-coherent spectral data. The intrinsic fluorescent spectral components are extracted from the coherent and non-coherent spectral data.
    Type: Grant
    Filed: October 18, 2019
    Date of Patent: June 2, 2020
    Assignee: Center for Quantitative Cytometry
    Inventors: Abraham Schwartz, Frank Mandy
  • Patent number: 10652484
    Abstract: The invention provides a method of obtaining data cubes such that elimination of the irrelevant portion of the illumination reveals the low intensity intrinsic spectral components. The method obtains target and reference data cubes using a single camera and of a single field of view so that low-level intrinsic spectral components may be detected and enhanced under conditions of high illumination intensity without the use of filters. Two exposures in rapid succession of the same field of view at the same camera settings are taken, one exposure of the target is taken under coherent conditions where the field of view is in focus, and the second exposure is of the same field of view taken under non-coherent conditions where the target is defocused such that it has no discernible spatial features in the field of view.
    Type: Grant
    Filed: October 18, 2019
    Date of Patent: May 12, 2020
    Assignee: Center for Quantitative Cytometry
    Inventors: Abraham Schwartz, Philip Sherman, Adolfas Gaigalas, Peter Ingram
  • Patent number: 10337916
    Abstract: A method is provided to obtain a full range intrinsic spectral signature for spectroscopy and spectral imaging. The method eliminates the irrelevant spectral components and is used to normalize the spectral intensities across the full wavelength ranges obtained from different instrumentation. The method determines the intrinsic instrument noise levels and the noise level across the spectral range is averaged for each spectrum. By determining the percent of the integrated instrument noise relative to the integrated illumination energy for each instrument, the instrument noise can be normalized to one common value and the intensity values of the intrinsic sample spectra can be normalized proportionately and combined into a continuous intrinsic spectrum across the wavelength ranges of the contributing instruments. The methodology is also implemented in spectral imaging and spectral data cubes.
    Type: Grant
    Filed: April 10, 2018
    Date of Patent: July 2, 2019
    Assignee: Center for Quantitative Cytometry
    Inventors: Abraham Schwartz, Adolfas K. Gaigalas, Peter Ingram
  • Patent number: 9998636
    Abstract: The present invention is a method of removing the illumination and background spectral components thus isolating spectra from multi-spectral and hyper-spectral data cubes. The invention accomplishes this by first balancing a reference and sample data cubes for each spectra associated with each location, or pixel/voxel, in the spatial image. The set of residual spectra produced in the balancing step is used to obtain and correct a new set of reference spectra that is used to remove the illumination and background components in a sample data cube.
    Type: Grant
    Filed: May 6, 2017
    Date of Patent: June 12, 2018
    Assignee: Center for Quantitative Cytometry
    Inventors: Abraham Schwartz, Philip Sherman, Peter Ingram
  • Patent number: 9435687
    Abstract: The present invention is a method of obtaining and isolating sample spectra from a wide wavelength illumination source without the use of filters. The method obtains the combined sample and illumination spectra of a sample and removes the illumination spectrum from the combined spectrum. This is accomplished by obtaining both the combined sample/illumination spectrum and the illumination spectrum separately at the same time and under the same environmental and instrument conditions. The illumination spectrum is then subtracted, wavelength by wavelength from the combined sample/illumination spectrum, leaving the pure sample spectrum which may a single spectrum or combination of two or more spectra from different types and/or compounds in the sample.
    Type: Grant
    Filed: October 6, 2014
    Date of Patent: September 6, 2016
    Assignee: CENTER FOR QUANTITATIVE CYTOMETRY
    Inventors: Abraham Schwartz, Martin C. Cohen, Peter Ingram