Abstract: The present invention relate to a highly efficient and novel method, using clean technologies, for obtaining a natural bioactive concentrate that is rich on polyphenols from olive mill water (OMW). The clean technologies integrate centrifugation, a drowning-out crystallization-based separation process, and vacuum evaporation. The method provides a highly-concentrated polyphenol isolate (up to 99% (mass fraction)) from other components presents in OMW, with up to half of the polyphenol content being hydroxytyrosol. The isolated polyphenols exhibit anti-oxidant, anti-microbial, anti-inflammatory, and anti-carcinogenic activities; they can be prepared as solid particles, as an aqueous solution, in an emulsion, or as lipidic-based nanoparticles. The isolated polyphenols can be used in the food industry, cosmetic industry, or pharmaceutical industry.

Abstract: The present invention relate to a highly efficient and novel method, using clean technologies, for obtaining a natural bioactive concentrate that is rich on polyphenols from olive mill water (OMW). The clean technologies integrate centrifugation, a drowning-out crystallization-based separation process, and vacuum evaporation. The method provides a highly-concentrated polyphenol isolate (up to 99% (mass fraction)) from other components presents in OMW, with up to half of the polyphenol content being hydroxytyrosol. The isolated polyphenols exhibit anti-oxidant, anti-microbial, anti-inflammatory, and anti-carcinogenic activities; they can be prepared as solid particles, as an aqueous solution, in an emulsion, or as lipidic-based nanoparticles. The isolated polyphenols can be used in the food industry, cosmetic industry, or pharmaceutical industry.