Abstract: Disclosed is a method for preparing an implant including a culture of cells on a membrane, the method including steps that consist of: securing the membrane to a mounting; placing the membrane in a recess of a housing providing two spaces having adjusted heights, above and below the membrane; injecting, into the spaces, a liquid capable of transforming into gel at a transport temperature lower than an injection temperature of the liquid; and bringing the housing to the transport temperature, so as to form an implant including the membrane and two layers of gel having adjusted thicknesses, on two opposite faces of the membrane, respectively.

Abstract: The invention provides a method for selecting pharmaceutical compounds that enhance inhibition of mevalonate synthesis in which mesodermal stem cells (MSC type) that are derived from human pluripotent cells or from induced stem cell are contacted with pharmaceutical compounds to be tested in the presence of an inhibitor of mevalonate synthesis. Pharmaceutical compounds are then identified which enhance cell toxicity in the presence of the inhibitors.

Abstract: The invention provides a method for selecting pharmaceutical compounds affecting mevalonate or cholesterol. The method having a step for putting into contact with the pharmaceutical compounds to be tested, cells of the MSC type obtained by a method for producing cells of the MSC type from human pluripotent cells or from induced stem cells, including a step for cultivating human pluripotent cells or induced stem cells in a culture medium of: 1) one or more growth factors selected from FGFs, HGF, PDGFs, EGF, herugulins and VEGFs; and 2) one or more antioxidants selected from ascorbic acid and its derivatives, vitamin E and N-acetylcysteine.

Abstract: A method for selecting pharmaceutical compounds affecting mevalonate or cholesterol, comprising a step for putting into contact with the pharmaceutical compounds to be tested, cells of the MSC type obtained by a method for producing cells of the MSC type from human pluripotent cells or from induced stem cells, comprising a step for cultivating human pluripotent cells or induced stem cells in a culture medium comprising: 1) One or more growth factors selected from FGFs, HGF, PDGFs, EGF, herugulins and VEGFs 2) One or more antioxidants selected from ascorbic acid and its derivatives, vitamin E and N-acetylcysteine.