Patents Assigned to Centre for Bioseparation Technology - VIT
  • Methods for producing recombinant factor VIII chains from non-filamentous fungi, their functional reconstitution and applications thereof
    Patent number: 9708387
    Abstract: A process of producing heavy chain peptide and/or light chain peptide of recombinant Factor VIII protein includes using the Pichia pastoris expression system. A process of producing a functional recombinant Factor VIII protein by reconstituting the Heavy chain and Light chain produced using said Pichia pastoris expression system. The said functional recombinant Factor VIII protein shows improved activity and therefore is used in the management of haemophilia.
    Type: Grant
    Filed: September 11, 2013
    Date of Patent: July 18, 2017
    Assignee: CENTRE FOR BIOSEPARATION TECHNOLOGY-VIT
    Inventors: Vijayalakshmi Mookambeswaran Arunachalam, Vignesh Narasimhan Janakiraman, Krishnan Venkataraman, Sudheer Reddy Aswatha Reddy, Satheeshkumar Padikkara Kutty, Sukesh Chandran Nair, Alok Srivastava
  • MONOLITH-BASED PSEUDO-BIOAFFINITY PURIFICATION METHODS FOR FACTOR VIII AND APPLICATIONS THEREOF
    Publication number: 20150315263
    Abstract: The present disclosure relates to purification of Factor VIII protein and/or its fragments from various sources by employing monolith based pseudobioaffinity purification methods. In particular, L-histidine over CIM monolith [Histidine Ligand Affinity Chromatography (HLAC)] is used for the purification of wild-type factor VIII from plasma cryoprecipitate, recombinant B-domain deleted factor VIII (rBDD-FVIII) expressed in various host systems and recombinant factor VIII light chain expressed in Pichia pastoris. Further, immobilized metal over CIM monolith [Immobilized metal-ion affinity chromatography (IMAC)] is employed for the purification of wild-type factor VIII, rBDD-FVIII expressed in various host systems and recombinant factor VIII heavy chain expressed in Pichia pastoris.
    Type: Application
    Filed: November 27, 2013
    Publication date: November 5, 2015
    Applicant: Centre for Bioseparation Technology - VIT
    Inventors: Vignesh Narasimhan Janakiraman, Rajaasekar Rajagopal Prasanna, Agamudi Sivasankaran Kamalanathan, Mookambeswaran Arunachalam Vijayalakshmi