Abstract: A process is disclosed for the long term surviving culture of hepatocytes maintaining their morphological and functional characteristics for long-term-periods, suitable to assest the acute and chronic hepatotoxicity of drugs, chemicals and environmental pollutants. The long term surviving culture of hepatocytes is carried out in a culture media containing fibroblast cells or fibroblast cell products treated to prevent cell multiplication and inoculated under controlled density.
Type:
Grant
Filed:
November 15, 1989
Date of Patent:
July 9, 1991
Assignee:
Centro de Investigacion y Estudios Avanzados del Instituto Politecnico Nacional
Inventors:
Walid Kuri-Harcuch, Tomas Mendoza-Figueroa