Abstract: The invention relates to Tandem Ubiquitin Affinity Entities (TUBES) which are fusion polypeptides comprising at least two ubiquitin binding domains connected to each other by non-naturally occurring spacer regions. The TUBES are capable of binding with high affinity to ubiquitin and to ubiquitylated polypeptides and are therefore suitable for the purification of ubiquitylated polypeptides from cellular extracts as well as for the identification of new ubiquitylated polypeptides.
Type:
Grant
Filed:
February 20, 2009
Date of Patent:
May 6, 2014
Assignee:
Centro de Investigation Cooperativa en Biociencias—CIC BIOGUNE
Inventors:
Manuel Salvador Rodriguez Medina, Roland Hjerpe