Abstract: Aspects of the present disclosure relate to methods and compositions for sugarcane transformation using morphogenic genes. In particular, the present disclosure relates to the use of morphogenic sequences that improve the efficiency of sugarcane cell or tissue transformation and genomic modification. The present disclosure further relates to genetically altered sugarcane cells, tissues, and plants produced using these methods and compositions.

Abstract: An industrial process using kestose hydrolases to enable the use of sugar mixtures containing 1-kestose in the industrial production of sucrose (as crystallized sugar) by providing a method for the conversion of 1-kestose into sucrose and fructose in a sugar solution, containing kestose and more than 10 mM sucrose (3 g/L), and comprising the enzymatic hydrolysis (preferably using 1-FEH enzymes—EC 3.2.1.153) of 1-kestose. The process further provides a method of producing a polypeptide having 1-kestose hydrolase activity and a composition comprising the polypeptide.

Abstract: The use of stillage in a saccharification/fermentation process of lignocellulosic biomasses, regardless of the form of the biomasses and regardless of the use of the obtained final hydrolyzed broth. The beneficial effect conferred by the stillage to the saccharification process of lignocellulosic biomasses presents among other characteristics the ability to buffer the reaction medium, especially when such process takes place in an enzymatic route, but not limited to it, regardless of the type of biomass being used and the type of pretreatment to which the biomass is subjected. Fermentation processes, such as the ethanol production, using stillage as source of nutrients such as, for example, nitrogen, for growing microorganisms, but not limited to it.

Abstract: Modern sugarcane cultivars are complex hybrids resulting from crosses among several species of the Saccharum genus. Traditional breeding methods have been extensively employed in different countries along the past decades to develop varieties with increased sucrose yield, and resistant to plagues and diseases. Conventional varietal improvement is, however, limited by the narrow pool of suitable markers. In this sense, molecular genetics is seen as a promising tool to assist in the process of molecular marker identification. The present invention concerns the identification of 348 genes associated with sucrose content in sugarcane plants. The genes were found to be differentially expressed when high sucrose and low sucrose plants and populations of plants were compared and/or when high and low sucrose internodes were compared. The expression data was obtained using cDNA microarray and quantitative PCR technologies.

Abstract: The use of stillage in a saccharification/fermentation process of lignocellulosic biomasses, regardless of the form of the biomasses and regardless of the use of the obtained final hydrolyzed broth. The beneficial effect conferred by the stillage to the saccharification process of lignocellulosic biomasses presents among other characteristics the ability to buffer the reaction medium, especially when such process takes place in an enzymatic route, but not limited to it, regardless of the type of biomass being used and the type of pretreatment to which the biomass is subjected. Fermentation processes, such as the ethanol production, using stillage as source of nutrients such as, for example, nitrogen, for growing microorganisms, but not limited to it.

Abstract: Modern sugarcane cultivars are complex hybrids resulting from crosses among several species of the Saccharum genus. Traditional breeding methods have been extensively employed in different countries along the past decades to develop varieties with increased sucrose yield, and resistant to plagues and diseases. Conventional varietal improvement is, however, limited by the narrow pool of suitable markers. In this sense, molecular genetics is seen as a promising tool to assist in the process of molecular marker identification. The present invention concerns the identification of 348 genes associated with sucrose content in sugarcane plants. The genes were found to be differentially expressed when high sucrose and low sucrose plants and populations of plants were compared and/or when high and low sucrose internodes were compared. The expression data was obtained using cDNA microarray and quantitative PCR technologies.

Abstract: Modern sugarcane cultivars are complex hybrids resulting from crosses among several species of the Saccharum genus. Traditional breeding methods have been extensively employed in different countries along the past decades to develop varieties with increased sucrose yield, and resistant to plagues and diseases. Conventional varietal improvement is, however, limited by the narrow pool of suitable markers. In this sense, molecular genetics is seen as a promising tool to assist in the process of molecular marker identification. The present invention concerns the identification of 348 genes associated with sucrose content in sugarcane plants. The genes were found to be differentially expressed when high sucrose and low sucrose plants and populations of plants were compared and/or when high and low sucrose internodes were compared. The expression data was obtained using cDNA microarray and quantitative PCR technologies.

Abstract: Modern sugarcane cultivars are complex hybrids resulting from crosses among several species of the Saccharum genus. Traditional breeding methods have been extensively employed in different countries along the past decades to develop varieties with increased sucrose yield, and resistant to plagues and diseases. Conventional varietal improvement is, however, limited by the narrow pool of suitable markers. In this sense, molecular genetics is seen as a promising tool to assist in the process of molecular marker identification. The present invention concerns the identification of 348 genes associated with sucrose content in sugarcane plants. The genes were found to be differentially expressed when high sucrose and low sucrose plants and populations of plants were compared and/or when high and low sucrose internodes were compared. The expression data was obtained using cDNA microarray and quantitative PCR technologies.