Abstract: A method of separation of sperm cells with undamaged intact heads from sperm cells with damaged heads and/or somatic cells and/or cell debris in a sperm sample obtained from a human, comprising the steps of: providing an anti-CD46 antibody or CD46-binding ligand bound to a carrier and/or to a tag; contacting the sperm sample with the anti-CD46 antibody or CD46 -binding ligand bound to the carrier and/or to the tag in order to bind CD46 presenting cells and/or cell debris from the sperm sample to the anti-CD46 antibody or CD46-binding ligand; removing the CD46 presenting cells bound via the anti-CD46 antibody or CD46 -binding ligand to the carrier and/or to the tag to obtain a sperm sample free of CD46 presenting cells, wherein CD46 presenting cells include sperm cells with damaged heads, somatic cells and cell debris, is disclosed.
Type:
Application
Filed:
January 17, 2021
Publication date:
February 9, 2023
Applicants:
BIOTECHNOLOGICKY USTAV AV CR, V. V. I., PRIMECELL BIOSCIENCE, A.S., CESKA ZEMEDELSKA UNIVERZITA V PRAZE
Inventors:
Katerina KOMRSKOVA, Pavla POSTLEROVA, Michaela FROLIKOV A, Serhiy FOROSTYAK, Ondrej SIMONIK
Abstract: Media, kits, and methods for cultivation of mammalian embryos from fertilized oocyte stage to blastocyst stage in the presence of N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine. The use of this supplement improves the early embryonic development, in particular the quality of zygotes and the blastocyst formation. The supplemented media can be used with pigs, humans, or other mammals N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine may be added to known media, and used for with vitro fertilization methods.
Type:
Grant
Filed:
June 8, 2017
Date of Patent:
November 26, 2019
Assignees:
Lekarska fakulta v Plzni, Univerzita Karlova (Faculty of Medicine in Pilsen, Charles University), The Curators of the University of Missouri, Chonbuk National University, Division of Biotechnology, College of Environmental and Bioresources, Ceska zemedelska univerzita v Praze, Institute of Animal Science
Inventors:
Jan Nevoral, Young-Joo Yi, Katerina Zamostna-Adamkova, Jaroslav Petr, Peter Sutovsky, Milena Kralickova
Abstract: Media, kits, and methods for cultivation of mammalian embryos from fertilized oocyte stage to blastocyst stage in the presence of N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine. The use of this supplement improves the early embryonic development, in particular the quality of zygotes and the blastocyst formation. The supplemented media can be used with pigs, humans, or other mammals N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine may be added to known media, and used for with vitro fertilization methods.
Type:
Application
Filed:
June 8, 2017
Publication date:
December 13, 2018
Applicants:
Lekarska fakulta v Plzni, Univerzita Karlova (Faculty of Medicine in Pilsen, Charles Univ), The Curators of the University of Missouri, Chonbuk National Univ, Division of Biotechnology, College of Environmental and Bioresources, Ceska zemedelska univerzita v Praze, Institute of Animal Science
Inventors:
Jan Nevoral, Young-Joo Yi, Katerina Zamostna-Adamkova, Jaroslav Petr, Peter Sutovsky, Milena Kralickova