Abstract: The present invention relates to a method for producing embryonic stem cell-derived cardiomyocytes, cardiomyocytes produced by the method, a method for producing cardiomyocyte bodies from the cardiomyocytes, cardiomyocyte bodies produced by the method, a cellular therapeutic agent comprising the cardiomyocyte bodies as an active ingredient for the treatment of cardiac diseases, a method for treating cardiac diseases using the cellular therapeutic agent, and use of cardiomyocytes or cardiomyocyte bodies for the preparation of the cellular therapeutic agent. The method for producing cardiomyocytes of the present invention can be used to easily purify differentiated cardiomyocytes from embryonic stem cells. Further, the purified cardiomyocytes can be used to produce cardiomyocyte bodies, which can be used as a cellular therapeutic agent for treating cardiac diseases. Therefore, the cardiomyocyte bodies can be widely applied to the development of prophylactic or therapeutic agents for cardiac diseases.
Abstract: Disclosed is a quickly soluble oral film dosage for masking a nasty taste, in particular, a quickly soluble oral film dosage comprising a stevioside based sweetener and a high potency sweetener in a ratio by weight (w/w) of 1:3 to 3:1, which may efficiently mask a bitter or nasty taste of a medicine and may be quickly dissolved in a mouth without water, thereby improving an aftertaste thereof thus enhancing dosage acceptability of a patient.
Type:
Grant
Filed:
August 10, 2012
Date of Patent:
November 15, 2016
Assignee:
CHABIO & DIOSTECH CO., LTD.
Inventors:
Jin-Kyu Park, Won-Suk Yang, Kyoung Tae Jung
Abstract: The present invention relates to a method for producing embryonic stem cell-derived cardiomyocytes, cardiomyocytes produced by the method, a method for producing cardiomyocyte bodies from the cardiomyocytes, cardiomyocyte bodies produced by the method, a cellular therapeutic agent comprising the cardiomyocyte bodies as an active ingredient for the treatment of cardiac diseases, a method for treating cardiac diseases using the cellular therapeutic agent, and use of cardiomyocytes or cardiomyocyte bodies for the preparation of the cellular therapeutic agent. The method for producing cardiomyocytes of the present invention can be used to easily purify differentiated cardiomyocytes from embryonic stem cells. Further, the purified cardiomyocytes can be used to produce cardiomyocyte bodies, which can be used as a cellular therapeutic agent for treating cardiac diseases. Therefore, the cardiomyocyte bodies can be widely applied to the development of prophylactic or therapeutic agents for cardiac diseases.
Abstract: Disclosed is a quickly soluble oral film dosage for masking a nasty taste, in particular, a quickly soluble oral film dosage comprising a stevioside based sweetener and a high potency sweetener in a ratio by weight (w/w) of 1:3 to 3:1, which may efficiently mask a bitter or nasty taste of a medicine and may be quickly dissolved in a mouth without water, thereby improving an aftertaste thereof thus enhancing dosage acceptability of a patient.
Type:
Application
Filed:
August 10, 2012
Publication date:
February 14, 2013
Applicant:
CHABIO & DIOSTECH CO., LTD.
Inventors:
Jin-Kyu Park, Won-Suk Yang, Kyoung Tae Jung
Abstract: Disclosed is a quickly soluble oral film dosage for masking a nasty taste, in particular, a quickly soluble oral film dosage comprising a stevioside based sweetener and a high potency sweetener in a ratio by weight (w/w) of 1:3 to 3:1, which may efficiently mask a bitter or nasty taste of a medicine and may be quickly dissolved in a mouth without water, thereby improving an aftertaste thereof thus enhancing dosage acceptability of a patient.
Type:
Application
Filed:
June 22, 2010
Publication date:
June 21, 2012
Applicant:
CHABIO & DIOSTECH CO. LTD
Inventors:
Jin-Kyu Park, Won-Suk Yang, Kyoung Tae Jung
Abstract: Provided are methods for the vitrification of human oocytes, which comprises: (a) placing human oocytes on a transfer instrument; and (b) placing the transfer instrument and the human oocytes directly into a slushed nitrogen (N2 slush), wherein the human oocytes are directly exposed to the N2 slush thereby undergoing vitrification, and wherein the human oocytes are able to live for a period of time after the human oocytes are devitrified.
Type:
Grant
Filed:
April 17, 2006
Date of Patent:
May 24, 2011
Assignees:
Chabio&Diostech Co. Ltd., College of Medicine Pochon Cha University Industry-Academic Cooperation Foundation
Inventors:
Tae Ki Yoon, Dong Ryul Lee, Hyung Min Chung, Kwang Yul Cha
Abstract: The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.
Type:
Grant
Filed:
June 13, 2007
Date of Patent:
April 5, 2011
Assignees:
Chabio & Diostech Co., Ltd., College of Medicine Pochon Cha University Industry-Academic Cooperation Foundation
Inventors:
Hyung-Min Chung, Sung-Hwan Moon, Ju-Mi Kim, Soo-Hong Lee
Abstract: The present invention provides a process for differentiation of vascular endothelial progenitor cells from embryoid bodies derived from embryonic stem cells, the process comprising: (a) treating a culture medium comprising embryoid bodies derived from embryonic stem cells such that the concentration of oxygen dissolved in the culture medium is in the range of about 1 ppm to about 5 ppm; (b) culturing the culture medium prepared in step (a) in an incubator in which the oxygen (O2) tension is equal to or less than about 15% to differentiate the embryoid bodies into vascular endothelial progenitor cells; and (c) isolating the vascular endothelial progenitor cells from the culture medium obtained in step (b).
Type:
Application
Filed:
August 14, 2008
Publication date:
September 2, 2010
Applicants:
CHABIO & DIOSTECH CO., LTD., COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION,
Inventors:
Hyung-Min Chung, Ju-Mi Kim, Soo-Hong Lee, Sung-Hwan Moon
Abstract: The present invention provides a method for isolating mesenchymal stem cells derived from a placental chorionic plate membrane, the method including: (a) harvesting a chorionic plate membrane from a detached placenta; (b) harvesting cells present in the chorionic plate membrane obtained in step (a) by scraping; (c) adding a solution containing trypsin and ethylenediaminetetraacetate to the cells obtained in step (b) to perform an enzymatic reaction and adding a fetal bovine serum thereto to terminate the enzymatic reaction; and (d) centrifuging the reaction solution obtained in step (c) and culturing the obtained cells in a medium containing a fetal bovine serum and an antibiotic.
Type:
Application
Filed:
October 24, 2007
Publication date:
May 6, 2010
Applicants:
CHABIO & DIOSTECH CO., LTD., COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY
Inventors:
Gi-Jin Kim, Kyung-Sun Shin, Kyu-Hwan Na, Hyung-Min Chung