Abstract: Disclosed is a polymer matrix based on a copolymer of acrylamide to immobilize antibodies in the radioimmunological determination of hormones and the pharmaceuticals. The specific copolymers are formed by polymerization of acrylamide and one or more copolymerizable compounds such as acrylic acid, methacrylic acid, methacrylamide, their derivatives and salts of acrylic acid or methacrylic acid.

Abstract: Disclosed is a method for the determination of unbound hormones and pharmaceuticals comprising placing a solution containing unbound hormones, hormones bound to bonding proteins and bonding proteins into contact with an immobilized antibody; reacting the unbound hormone with the antibody; eluting the hormones bound to bonding proteins and the bonding hormones with a solution containing a marked hormone; reacting the marked hormone with the antibody; eluting the marked hormone not reacted with the antibody, and determining the marked hormone by radioimmunological evaluation.

Abstract: Disclosed is a method for the total determination of hormones and pharmaceuticals partially bound to specific or non-specific proteins, by the enzymatic hydrolysis of the bonding proteins, the reaction of the hormones or pharmaceuticals with an antibody and the radioimmunological determination of the hormones and pharmaceuticals, characterized by the fact that the immobilized antibodies are used.

Abstract: Disclosed is a multichannel system for the handling of immobilized biologically active substance, in which several test tubes are filled with at least one reaction liquid. The automated handling of a large number of samples in the shortest possible time is made possible by this system.