Abstract: A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil-water mixture is provided, wherein the oil-water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinant Candida rugosa lipase 1 (rCRL1) is added into the oil-water mixture. (iii) The oil-water mixture is emulsified. (iv) The emulsified oil-water mixture is hydrolyzed and fatty acid is generated. (v) Oil-water is separated at a temperature of 55° C. to 65° C. and an oil phase layer is extracted. (vi) The cooling and filtering step is performed to obtain cis-unsaturated fatty acid.
Type:
Grant
Filed:
April 14, 2021
Date of Patent:
June 27, 2023
Assignees:
CHANT OIL CO., LTD., National Taiwan Normal University
Inventors:
Guan-Chiun Lee, Ting-Chun Kuo, Shun-Niang Chen, Chia-Chi Lin
Abstract: The present invention relates to a biowax comprising a partial acyl glyceride selected from the group consisting of a monoacylglyceride, a diacylglyceride and the combination thereof. The present invention also relates to a biocandle comprising a biowax and a wick, and to a method of producing the same.