Abstract: In a device for studying and recording electrophysiological phenomena in culture of excitable tissues comprising a lower half card with a perfusion membrane in a perfusion chamber sealed by a permeable and transparent membrane and an upper half card with a flexible printed circuit bearing a network of electrode and fitted with a cap for insuring the closure of the device, the improvement comprising the upper half card being comprised of an upper half and a lower half card which when assembled together for recording yield a functional set up.

Abstract: A device for culturing dissociated cultures or organotypic cultures on pieces of pre-cut membrane comprising discs of membrane which are circled by a plastic ring provided with a tab and the patch of membrane which is placed in a culture medium selected from the group consisting of in a culture insert, on a semi-solid medium and on the membrane of a box, consisting of a case made of transparent plastic material formed by two assembled parts which are hermetically sealed, the lower part being separated into two compartments by a permeable membrane defining a lower compartment which serves as a reservoir for the culture medium and an upper compartment intended to receive one or more circled membrane discs or eyelets, serving as the site for the cell culture and its use.

Abstract: Device for studying and recording the electrophysiological phenomena in cultures of excitable tissues, said device comprising a combination of a lower half card comprising a perfusion medium in a perfusion chamber sealed by a permeable and transparent membrane and an upper half card with a flexible printed circuit bearing a network of electrodes and fitted with a cap for insuring the closure of the device and a method of use thereof.