Abstract: Polynucleotide primers and probes for the specific detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in samples are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin. The primers and probes can be used in real-time diagnostic assays for rapid detection of E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin in a variety of situations and are capable of distinguishing E. coli 0157:H7 and/or E. coli 0157:NM which produces verotoxin from other E. coli strains. Kits comprising the primers and probes are also provided.
Abstract: A system and method for testing for microbial contamination using a digital flow cytometer comprising an illumination source, a fluorescence detector, a digitizing device, and a digitized signal transferring device.
Type:
Grant
Filed:
August 14, 2000
Date of Patent:
April 24, 2001
Assignee:
Chemunex S.A.
Inventors:
Warren Groner, Jaspal Sanghera, Patrick Desfetes