Abstract: A one time programmable OTP memory array and a read and write method thereof are provided. The OTP memory array includes M×N OTP memories, the OTP memories each include a storage MOS transistor, a first MOS transistor, a second MOS transistor and a detection MOS transistor, an isolation module is disposed between a control terminal of the detection MOS transistor and the storage MOS transistor; the isolation module includes at least one isolation MOS transistor; and in the array, a gate of each storage MOS transistor is connected to a same storage control point, each isolation MOS transistor is distinguished based on a distance from the storage MOS transistor, and gates of isolation MOS transistors with a same distance from the storage MOS transistor are connected to a same isolation control point.