Abstract: A method and apparatus for time-resolved fluorescence spectroscopy is described in which laser light from a single pulse is used to excite fluorescent photons in a sample, which fluorescence is detected by a PMT optimized for linearity and response time to produce photoelectrons which generate a current at the PMT anode. This current is discharged through an R/C network to produce a voltage amplitude waveform which is converted to an optical image, intensified, stored and digitized. The digitized version of the optical image is processed in a data processor to calculate the true fluorescence impulse response.
Type:
Grant
Filed:
March 27, 1987
Date of Patent:
August 8, 1989
Assignee:
Chimerix Corporation
Inventors:
Yong-Sheng Chao, Salvador M. Fernandez, Ernest F. Guignon