Abstract: The present disclosure discloses a whole avian-origin reverse genetic system, a recombinant H5N2 subtype avian influenza virus, a vaccine containing the virus, and a preparation method and application thereof. The genome of the recombinant virus is comprised of a modified HA gene derived from a highly pathogenic H5N6 subtype avian influenza virus strain, as well as PB2, PB1, PA, NP, NA, M and NS genes derived from H5N2 subtype avian influenza D7 virus strain. The recombinant virus is a recombinant H5N2 avian influenza virus rescued from the D7 virus strain as a backbone, which is an avirulent virus strain with the original immunogenicity, and can maintain a high virus titer during the chick embryo culture process. The recombinant virus fully meets the biological safety requirements and has a good application prospect.

Abstract: The present disclosure discloses a whole avian-origin reverse genetic system, a recombinant H5N2 subtype avian influenza virus, a vaccine containing the virus, and a preparation method and application thereof. The genome of the recombinant virus is comprised of a modified HA gene derived from a highly pathogenic H5N6 subtype avian influenza virus strain, as well as PB2, PB1, PA, NP, NA, M and NS genes derived from H5N2 subtype avian influenza D7 virus strain. The recombinant virus is a recombinant H5N2 avian influenza virus rescued from the D7 virus strain as a backbone, which is an avirulent virus strain with the original immunogenicity, and can maintain a high virus titer during the chick embryo culture process. The recombinant virus fully meets the biological safety requirements and has a good application prospect.

Abstract: The present disclosure discloses a whole avian-origin reverse genetic manipulation system and its use in producing a recombinant H7N9 avian influenza vaccine. The whole avian-origin reverse genetic manipulation system is an eight-plasmid reverse genetic manipulation system based on H5N2 subtype avian influenza D7 virus strain, which is comprised of 8 recombinant plasmids respectively containing PB2, PB1, PA, HA, NP, NA, M and NS gene fragments derived from H5N2 subtype avian influenza D7 virus strain. The genome of the recombinant H7N9 subtype avian influenza vaccine of the present disclosure is comprised of an NA gene and a modified HA gene derived from a highly pathogenic H7N9 subtype avian influenza virus strain, as well as PB2, PB1, PA, NP, M and NS genes derived from H5N2 subtype avian influenza D7 virus strain.

Abstract: The present invention relates to a photo-induced cationic polymerized pure vegetable oil-based polymer and a preparation method and use thereof. The preparation method comprises the following steps: mixing a dry oil, an epoxy vegetable oil and an initiator uniformly, irradiating to initiate a photo-curing reaction, and then placing at ambient temperature, and continuing to a heat-curing reaction so as to obtain a photo-induced cationic polymerized pure vegetable oil-based polymer. In the present invention, vegetable oil resources which are low in price, widespread, and easy to regenerate are used to prepare the pure vegetable oil-based polymers instead of the fossil-derived monomers completely, thereby achieving the efficient use of vegetable oils. In the present invention, an unconventional photo-induced heat frontal polymerization technology is used to prepare the pure vegetable oil-based polymer, thereby achieving a photo-thermal dual curing reaction of a vegetable oil system without heating.

Abstract: The present disclosure discloses design and forming method of a channel of flow-type thin wall drip irrigation belt. The method includes the steps of construction of a double-layer asymmetric channel structure, determination of a structure parameter control threshold and optimal value, design of water inlets and water outlets of the double-layer asymmetric channel structure, proposal of a machining method of a molding wheel matching the double-layer asymmetric structure and an optimization method of a flow-type drip irrigation belt molding process, material modification of a flow-type drip irrigation belt and design of a reasonable formulation, realization the stereotype production of the flow-type drip irrigation belt, etc.

Abstract: The present disclosure discloses a Microcin MccY, and a preparation method and use thereof, wherein the amino acid sequence of the Microcin MccY is GGRGHIAEYFSGPITQVSFYG. Compared with Microcin MccJ25 that only has a bactericidal activity against a small part of serotypes of Salmonella such as Salmonella enteritidis, the Microcin MccY has bacteriostatic/bactericidal effects on Salmonella Pullorum, Salmonella typhimurium, Salmonella kentucky, Salmonella Infantis, Salmonella London, and Shigella sonnei, and can kill other serotypes of Salmonella that MccJ25 cannot kill. Especially, the Microcin MccY has an outstanding bactericidal effect on Salmonella typhimurium and Salmonella Pullorum which are common in livestock and poultry production, and meanwhile it also has bacteriostatic/bactericidal effects on Shigella sonnei. Therefore, the Microcin MccY overcomes the shortcoming of the narrow spectrum of the Microcin, has breakthrough significance, and has the potential as a substitute to antibiotics.

Abstract: Compositions and methods for identifying, selecting and producing maize plants with enhanced ear rot resistance are provided. Ear rot resistance maize plants and germplasms are also provided. In some embodiments, methods of identifying an ear rot resistance maize plant or germplasm are provided. Such methods may comprise detecting, in the plant or germplasm, a marker associated with enhanced ear rot resistance.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.

Abstract: A fused tricyclic compound, an optical isomer, cis and trans isomers or an agromedically acceptable salt thereof, and insecticidal use thereof in agroforestry. A pyrazole-ring-contained fused heterocyclic compound, an agricultural composition, and a method of using the agricultural composition are also provided.

Abstract: The present disclosure discloses a Microcin MccY, and a preparation method and use thereof, wherein the amino acid sequence of the Microcin MccY is GGRGHIAEYFSGPITQVSFYG. Compared with Microcin MccJ25 that only has a bactericidal activity against a small part of serotypes of Salmonella such as Salmonella enteritidis, the Microcin MccY has bacteriostatic/bactericidal effects on Salmonella Pullorum, Salmonella typhimurium, Salmonella kentucky, Salmonella Infantis, Salmonella London, and Shigella sonnei, and can kill other serotypes of Salmonella that MccJ25 cannot kill. Especially, the Microcin MccY has an outstanding bactericidal effect on Salmonella typhimurium and Salmonella Pullorum which are common in livestock and poultry production, and meanwhile it also has bacteriostatic/bactericidal effects on Shigella sonnei. Therefore, the Microcin MccY overcomes the shortcoming of the narrow spectrum of the Microcin, has breakthrough significance, and has the potential as a substitute to antibiotics.

Abstract: The present disclosure provides a method for rapid preparation of an epidemic infectious bronchitis vaccine. An infectious clone of an infectious bronchitis virus (IBV) H120 vaccine strain is used as a skeleton carrier and an antigen gene in the skeleton carrier is replaced with a target antigen gene of an epidemic infectious bronchitis virus strain, to obtain a recombinant bronchitis virus. The target antigen gene is an S1 gene or an S gene. The S gene is one of the S gene fragments of the epidemic infectious bronchitis virus strain or a fusion gene composed of multiple S gene fragments. Furthermore, the target antigen gene and an N gene can be simultaneously replaced and a signal peptide region of the original S1 gene in the skeleton carrier is retained during the replacement.

Abstract: The present invention discloses a maize parthenogenetic haploid-inducing gene ZmPLA1E and application thereof. The present invention utilizes the method of exchanged individual plant progeny tests for the first time, and successfully proves that the ZmPLA1E gene can generate and significantly increase the parthenogenetic haploid induction ability in the process of self-crossing or hybridizing as a male parent with other maize materials after the ZmPLA1E gene is mutated in the coding region. The haploid-inducing gene ZmPLA1E of the present invention is important for the cultivation of high-frequency parthenogenetic haploid inducing lines and the application of haploid technology.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.

Abstract: The present invention discloses a method for increasing the sensitivity of a plant to a gibberellin inhibitor. The method for increasing the sensitivity of a plant to a gibberellin inhibitor provided by the present invention comprises the step of introducing an encoding gene of a protein into a recipient plant to obtain a transgenic plant; the transgenic plant has increased sensitivity to the gibberellin inhibitor compared to the recipient plant; the protein is a protein of the following a) or b) or c): a) a protein whose amino acid sequence is set forth in positions 1-821 of SEQ ID NO: 1; b) a protein whose amino acid sequence is set forth in SEQ ID NO: 1; c) a fusion protein obtained by attaching tag(s) to the N-terminus or/and C-terminus of a) or b).

Abstract: Embodiments of the present disclosure belongs to the technical field of animal feed and provides a method for rapidly evaluating metabolizable energy of goose diet by using a technique of simulative digestion gross energy. By using the technical means combining the biological method and the simulative digestion gross energy technique, metabolizable energy of goose feed can be evaluated quickly. Based on the “stomach-small intestine” two-step enzymatic methods, it is the first time to establish a regression equation between the metabolizable energy change and fiber level in the cecum to rectify the value of simulative digestion gross energy in the cecal microbial digestion phase, making the simulative digestion gross energy technique more reasonable in the assessment of metabolizable energy in geese. Results show that the use of simulative digestion gross energy technique to assess the metabolizable energy of goose feed value is highly feasible.

Abstract: Provided is use of Penicillium sclerotiorum SCAUMCX01, under accession number GDMCC No. 60249, in the preparation of plant growth regulators or inducers. The plant growth regulators or inducers containing Penicillium sclerotiorum, Penicillium sclerotiorum spores, a culture solution thereof and an extract of the culture solution have the effects of promoting the early germination of seeds, promoting the growth of the underground roots of crops and the branching of above-ground parts of crops, improving the bioavailability of crops, delaying the aging progress of crops, and enhancing the growth of crops.

Abstract: The present invention discloses a gradient slow-release active composite film and a preparation method thereof. The active composite film, from inside to outside, is composed of an antioxidative hygroscopic internal layer, at least one gradient anti-microbial antioxidative intermediate layer and at least one waterproof external layer; wherein the antioxidative hygroscopic internal layer is prepared by an alcohol-soluble protein-water-soluble protein substrate and a water-soluble antioxidant; the gradient anti-microbial antioxidative intermediate layer is prepared by an alcohol-soluble protein-water-soluble protein substrate and a lipid-soluble plant essential oil and a water-soluble antioxidant, wherein a mass ratio of the alcohol-soluble protein to the water-soluble protein is (1-2):(1-2); and the waterproof external layer is composed of a hydrophobic alcohol-soluble protein layer.

Abstract: A fused tricyclic compound, an optical isomer, cis and trans isomers or an agromedically acceptable salt thereof, and insecticidal use thereof in agroforestry. A pyrazole-ring-contained fused heterocyclic compound, an agricultural composition, and a method of using the agricultural composition are also provided.

Abstract: The present invention discloses an edible film with enhanced release efficiency of essential oil and a preparation method thereof. The edible film is composed of at least one blank layer and at least one essential oil-rich layer; during casting, the blank layer serves as a substrate layer and the essential oil-rich layer serves as an intermediate layer or an external layer; wherein the blank layer is composed of an alcohol-soluble protein-water-soluble protein composite film, and the essential oil-rich layer is composed of an alcohol-soluble protein-water-soluble protein composite film loading with essential oil. The present invention provides a structure and a method of divisional enrichment of essential oil, wherein the essential oil of equal mass is enriched in a certain layer of the film. Compared with the existing edible film loading with essential oil, the essential oil is loaded to the entire film matrix in the present invention.

Abstract: A new and distinct cultivar of Rhododendron plant named ‘Spring Mate’, characterized by its upright to broadly spreading and mounding plant habit; moderately vigorous growth habit and moderate growth rate; freely branching habit and dense and bushy appearance; dark green-colored leaves; freely flowering habit; ruffled semi-double flowers that are vivid reddish orange in color; and good garden performance and temperature tolerance.