Abstract: Provided are a method and a kit for constructing a simplified genomic library. The method comprises: performing a non-specific amplification on a whole genome with a first pair of primers to obtain random amplified fragments; performing a specific amplification on the random amplified fragments with a second pair of primers to obtain the simplified genomic library.

Abstract: A preparation method of a carbon-based selenium-enriched fertilizer for rice is disclosed. The preparation method of the carbon-based selenium-enriched fertilizer for rice includes planting a selenium-enriched plant material; collecting and concentrating a selenium-enriched extracting solution; preparing modified selenium-enriched biomass charcoal from the tertiary residues; and preparing the carbon-based selenium-enriched fertilizer. In the present disclosure, active organic selenium enriched and generated by plant is compounded with the biomass charcoal, so that the fixation and loss of the active selenium in soil are reduced, and the utilization efficiency of the selenium fertilizer is improved.

Abstract: Provided are a gene combination used for controlling foreign gene expression in a specific plant tissue, and a method applying the gene combination to cultivate a transgenic plant. The method is used to cultivate, for example, an endosperm zero expression-type transgenic rice, i.e., rice grain endosperm produced by the rice does not contain any transgenic product protein synthesis and accumulation.

Abstract: A organic fertilizer for controlling pollution or accumulation of heavy metal cadmium in rice and a preparation method and an application method thereof are related to the technical field of fertilizers. The fertilizer is, based on 100 kg of organic material with a water content of 15%, added with 0.05-5 kg of iron salt calculated as iron, and is also added with manganese salt, zinc salt and copper salt, wherein a mass ratio of the iron salt calculated as iron, the manganese salt calculated as manganese, the zinc salt calculated as zinc and the copper salt calculated as copper is 1: 0.2-8: 0.08-2: 0.001-0.5.

Abstract: A preparation method of a carbon-based selenium-enriched fertilizer for rice is disclosed. The preparation method of the carbon-based selenium-enriched fertilizer for rice includes planting a selenium-enriched plant material; collecting and concentrating a selenium-enriched extracting solution; preparing modified selenium-enriched biomass charcoal from the tertiary residues; and preparing the carbon-based selenium-enriched fertilizer. In the present disclosure, active organic selenium enriched and generated by plant is compounded with the biomass charcoal, so that the fixation and loss of the active selenium in soil are reduced, and the utilization efficiency of the selenium fertilizer is improved.

Abstract: Provided are a gene combination used for controlling foreign gene expression in a specific plant tissue, and a method applying the gene combination to cultivate a transgenic plant. The method is used to cultivate, for example, an endosperm zero expression-type transgenic rice. i.e., rice grain endosperm produced by the rice does not contain any transgenic product protein synthesis and accumulation.

Abstract: Provided are an artificially designed and modified insecticidal protein or a segment thereof. The insecticidal protein belongs to a Vip3A-type insecticidal protein. The protein or the segment thereof has an insecticidal activity against a pest, especially a lepidoptera pest. Also provided are a nucleic acid for coding the protein or the segment thereof, an insecticidal composition, a DNA constructor, and a transformed microorganism or a plant comprising the nucleic acid, and a method for controlling a pest, especially a plant pest.

Abstract: The present invention provides a soil heavy metal curing agent for controlling accumulation of heavy metals of crops and its preparation method. The curing agent is made from the following parts of raw materials by weight: 60˜140 parts of substance containing carbon-carbon double bond; 1˜400 parts of sulfo-compound by sulfur; 50˜500 parts of organic matter by 10% water content; 0˜400 parts of water; 0˜100 parts of an initiator; 0˜200 parts of a reducer; and 0˜200 parts of a strong base. The curing agent for heavy metals in the soil according to the present invention can reduce the cadmium, lead and mercury content in the soil and further greatly reduce the roots' absorption of these heavy metals.

Abstract: A noninvasive method of source-sink regulation in rice belongs to the technical field of rice production. In this method, the source-sink relationship is regulated by a rice sterile line and its identical type of maintaining line being subjected to mixed-planting and insulated pollination, or sowing and transplanting at different times and insulated pollination, so as to construct rice plant samples with gradient difference of source-sink levels. The present invention is a kind of native, natural noninvasive method of source-sink regulation, which could broaden the traditional thinking of source-sink theoretical research, especially overcome the deficiency in conventional methods such as leaf-cutting, spikelet-thinning that lead to physical injury or physiological interference.

Abstract: The present invention provides a soil heavy metal curing agent for controlling accumulation of heavy metals of crops and its preparation method. The curing agent is made from the following parts of raw materials by weight: 60˜140 parts of substance containing carbon-carbon double bond; 1˜400 parts of sulfo-compound by sulfur; 50˜500 parts of organic matter by 10% water content; 0˜400 parts of water; 0˜100 parts of an initiator; 0˜200 parts of a reducer; and 0˜200 parts of a strong base. The curing agent for heavy metals in the soil according to the present invention can reduce the cadmium, lead and mercury content in the soil and further greatly reduce the roots absorption of these heavy metals.

Abstract: The invention relates to a method for simplifying hybrid rice seed production procedure and improving the working efficiency of seed production. The method comprises the following steps: (1) breeding small grain CMS or GMS line and corresponding hybrid rice having characteristics of rice (Oryza sativa) ZH-sg (Zhonghua 11 small grain mutant) with the accession number of CGMCC No. 2741; (2) hybridizing the small grain CMS line with large grain or normal grain restorer line hybrid rice for seed producing; (3) carrying out mechanized seed production of mixing sowing and mixing harvesting by utilizing small grain CMS line hybrid rice; (4) seed cleaning and separation after mechanical harvest. The invention can save a large amount of seed production field, reduce the investment of labor force in seed production, simplify the operation procedure and reduce mechanical admixture, storage and transportation cost and buying cost of hybrid rice seeds for rice farmers.

Abstract: The invention relates to a method for simplifying hybrid rice seed production procedure and improving the working efficiency of seed production. The method comprises the following steps: (1) breeding small grain CMS or GMS line and corresponding hybrid rice having characteristics of rice (Oryza sativa) ZH-sg (Zhonghua 11 small grain mutant) with the accession number of CGMCC No. 2741; (2) hybridizing the small grain CMS line with large grain or normal grain restorer line hybrid rice for seed producing; (3) carrying out mechanized seed production of mixing sowing and mixing harvesting by utilizing small grain CMS line hybrid rice; (4) seed cleaning and separation after mechanical harvest. The invention can save a large amount of seed production field, reduce the investment of labor force in seed production, simplify the operation procedure and reduce mechanical admixture, storage and transportation cost and buying cost of hybrid rice seeds for rice farmers.