Abstract: An insect-resistant herbicide-tolerant corn transformation event, and a related creation method, a detection method, and application thereof are provided herein. Using the corn inbred line Xiang 249 as a receptor, by means of agrobacterium-mediated genetic transformation, obtaining a corn plant with an exogenous gene insert inserted at a specific genomic locus, the exogenous gene insert comprising the following three genes: an insect-resistant gene, a glufosinate resistant gene, and a glyphosate resistant gene. In the obtained transformation event, the inserted exogenous genes are positioned at a non-functional locus of the corn genome, and do not affect the expression of the other genes of the receptor plant, such that the transgenic corn plant maintains good agronomic traits whilst acquiring insect resistance and herbicide tolerance.

Abstract: An insect-resistant herbicide-tolerant corn transformation event, and a related creation method, a detection method, and application thereof are provided herein. Using the corn inbred line Xiang 249 as a receptor, by means of agrobacterium-mediated genetic transformation, obtaining a corn plant with an exogenous gene insert inserted at a specific genomic locus, the exogenous gene insert comprising the following three genes: an insect-resistant gene, a glufosinate resistant gene, and a glyphosate resistant gene. In the obtained transformation event, the inserted exogenous genes are positioned at a non-functional locus of the corn genome, and do not affect the expression of the other genes of the receptor plant, such that the transgenic corn plant maintains good agronomic traits whilst acquiring insect resistance and herbicide tolerance.

Abstract: Provided is a novel anti-armyworm use of cry1Ab/cry1AcZM gene. Said gene can be used for controlling or killing Mythimna separate (Walker) and reducing injury to plants by Mythimna separate.

Abstract: The present disclosure relates to a gene sequence Cry1Ab/Cry1AcZM, an expression cassette containing the gene, an expression vector, a host cell and the use thereof in plant breeding. The protein encoded by an insect-resistant gene according to the disclosure can be expressed in a monocotyledonous plant and further used for culturing an insect-resistant transgenic monocotyledonous plant harboring transgenes Cry1Ab/Cry1AcZM. Bioassay tests prove that the modified and synthesized gene sequence Cry1Ab/Cry1AcZM and the expression product of the constructed vector according to the disclosure have a killing effect on lepidopteran pests.

Abstract: The present disclosure relates to a gene sequence Cry1Ab/Cry1AcZM, an expression cassette containing the gene, an expression vector, a host cell and the use thereof in plant breeding. The protein encoded by an insect-resistant gene according to the disclosure can be expressed in a monocotyledonous plant and further used for culturing an insect-resistant transgenic monocotyledonous plant harboring transgenes Cry1Ab/Cry1AcZM. Bioassay tests prove that the modified and synthesized gene sequence Cry1Ab/Cry1AcZM and the expression product of the constructed vector according to the disclosure have a killing effect on lepidopteran pests.