Patents Assigned to Chiron Therapeutics
  • Process for purifying bacterially produced M-CSF
    Patent number: 5466781
    Abstract: A process is described for producing M-CSF from bacteria. It includes: fermentation of bacteria containing M-CSF DNA; harvest of the fractions that contain the M-CSF protein (refractile bodies); primary recovery of the protein; solubilization and denaturation of refractile bodies; M-CSF refolding; purification by column chromatography and other methods; and formulation of the properly refolded M-CSF. This method is advantageous over prior methods in terms of yield and purity.
    Type: Grant
    Filed: March 8, 1993
    Date of Patent: November 14, 1995
    Assignee: Chiron Therapeutics
    Inventors: Glenn Dorin, David R. Gray, Byeong S. Chang, Cynthia A. Cowgill, Robert J. Milley