Abstract: An intelligent microbial sample treatment system includes a workbench, and a sample treatment assembly, a culture medium treatment assembly, a streaking assembly and a culture medium storage assembly that are disposed on the workbench; the sample treatment assembly comprises a sample transfer device, a filling device, and a scanning device, a weight detection device, a filling location, a shaking device and waiting locations that are disposed in sequence; and the sample transfer device moves a sample cup among the scanning device, the weight detection device, the filling location, the shaking device and the waiting locations. The intelligent microbial sample treatment system disclosed by the present invention enables improvement of work efficiency and reduction of space occupation and has a reasonable structural layout and high work efficiency.