Abstract: A method of isolating a lactic acid bacterial DNA fragment comprising a promoter, the method comprising introducing a DNA molecule comprising a transposable element comprising a promoterless structural gene as a promoter probe gene into a population of a lactic acid bacterium, methods of constructing a recombinant lactic acid bacterium comprising a regulatable promoter by using the above method, a recombinant lactic acid bacterium comprising a gene coding for a desired gene product and operably linked thereto a regulatable lactic acid bacterial promoter not natively associated with the gene, the use of such a recombinant lactic acid bacterium and recombinant plasmids comprising a regulatable lactic acid bacterial promoter.
Type:
Grant
Filed:
March 1, 1995
Date of Patent:
November 17, 1998
Assignees:
Bioteknologisk Institut, CHR Hansen's Laboratorium Danmark A/S
Inventors:
Hans Israelsen, Egon Bech Hansen, Eric Johansen, Soeren Michael Madsen, Dan Nilsson, Astrid Vrang