Abstract: A bioanalytical method for determining catalases and peroxidases using europium ions.

Abstract: The present invention concerns biological methods of determination using enzymes from the group of oxidases and using optical indicators from the group of lanthanoid-ligand complexes.

Abstract: New reactive dyes are described which can be used to fluorescently label bioorganic molecules such as amino acids, proteins, antibodies, nucleotides and also polymer particles. The color of the new dyes and conjugates thereof can be varied over a wide range. In contrast to known symmetric cyanine dyes, the dyes of the invention contain only one reactive group. Hence the labelling takes place without the interfering cross-linking that occurs with bireactive dyes. Their fluorescence quantum yields are very high (especially in the conjugated form). Conjugates thereof with proteins, oligonucleotides or particles can be used in fluorescence-based analytical methods of determination e.g. in immunoassays, in hybridization assays, in cytometry or for pharmaceutical screening.

Abstract: In nanoparticles, a phosphorescent donor dyestuff and several fluorescent acceptor dyestuffs are immobilized together. These nanoparticles serve as multiplex marker for a number of analytes, which can be determined according to absorption spectra of the acceptor dyestuffs as well as according to the luminescence-decay period of the respective dyestuffs.