Abstract: An essential oil of stems and leaves of Chuzhou chrysanthemum and its applications are provided. The essential oil is prepared by: taking and crushing fresh stems and leaves of the Chuzhou chrysanthemum by a crusher; putting the crushed stems and leaves into an extraction kettle, adding deionized water according to a ratio of 1:4 to 1:6, sealing, and connecting a cooling reflux and essential oil collection device; heating and starting counting when boiling, performing distillation extraction; then standing for cooling to room temperature, naturally layering the essential oil and water, discharging the water at a lower layer, and collecting the essential oil at an upper layer; and placing the essential oil in a brown bottle, adding anhydrous sodium sulfate to absorb water to obtain the essential oil of the stems and leaves of Chuzhou chrysanthemum.