Abstract: The present invention provides a method for preparing an extract fraction reinforced with ginsenoside Rg1 or Rb1 from ginseng. The method for preparing an extract fraction reinforced with ginsenoside Rg1 comprises the steps of: concentrating an alcohol extract of ginseng and then adsorbing the extract diluted in water by adding the extract to an adsorption resin; passing distilled water through the adsorption resin, then eluting and removing unadsorbed ingredients; and adding 30 to 40 v/v % alcohol to the adsorption resin to obtain an eluate. The method for preparing an extract fraction reinforced with ginsenoside Rb1 comprises the steps of: concentrating an alcohol extract of ginseng and then adsorbing the extract diluted in water by adding the extract to an adsorption resin; passing distilled water through the adsorption resin, then eluting and removing unadsorbed ingredients; and adding 50 to 80 v/v % alcohol to the adsorption resin and then eluting.