Abstract: A stylet for use with an automatic tissue microarrayer is provided for arraying frozen tissue samples on a substrate. In one embodiment, the stylet comprises a stylet needle having a pushing surface for pushing either frozen tissue or embedding media out of a coring needle, and a connecting end. In another embodiment the stylet comprises a stylet body which comprises a stylet base for sliding along the length of the needle and a stylet cap for coupling to the connecting end of the stylet needle. In a further embodiment of the invention, the stylet base and stylet cap are separated from each other by a resilient element, such as a spring. Molds for generating component parts of the stylet are also provided.

Abstract: A device particularly suited for constructing frozen tissue microarrays. The device comprises: a cooling chamber for receiving at least one frozen material and for maintaining the frozen material in a frozen condition; the cooling chamber moveable in an x and y direction relative to a horizontal surface; at least one coring needle comprising a cutting surface and a lumen for receiving a core of frozen material cut by the cutting surface; and at least one coring needle positioning element, for positioning the at least one coring needle over said frozen material for cutting said frozen material. Frozen tissue microarray blocks and methods of generating these are also provided.

Abstract: A device particularly suited for constructing frozen tissue microarrays. The device comprises: a cooling chamber for receiving at least one frozen material and for maintaining the frozen material in a frozen condition; the cooling chamber moveable in an x and y direction relative to a horizontal surface; at least one coring needle comprising a cutting surface and a lumen for receiving a core of frozen material cut by the cutting surface; and at least one coring needle positioning element, for positioning the at least one coring needle over said frozen material for cutting said frozen material. Frozen tissue microarray blocks and methods of generating these are also provided.