Abstract: Polyallylamine conjugates and applications thereof for biological signal amplification are provided by utilizing the essential amino group of polyallylamine to covalently bind with capture agents and signal molecules having the functional groups selected from a group consisting of —NHS, —CO, —S?O2 and —C?O—C?O. The resulting conjugates having more than one signaling entities can be further implemented for biological expression with enhancing effect on biological signal intensity, such that the sensitivity of detection for the variation between biological interactions is largely increased.

Abstract: This invention is provided a biochip containing splitable reaction wells and method for producing same and application thereof. The invention avoids cross contamination or interference between different samples on the same biochip. In addition, the soft polymer film removed from reaction wells may be reused on different substrates, thereby saving the cost of experiment or clinical testing.

Abstract: The present invention provides a substrate for protein microarrays, whereby compound A and GPTS are mixed for coating onto a solid support to form a layer, wherein said compound A is selected from a group consisting of nitrocellulose, poly(styrene-co-maleic anhydride) and polyvinylidene fluoride. Moreover, the present invention also provides a protein microarray by depositing proteins on said layer of said substrate.

Abstract: A protein chip and the preparation thereof. The preparation includes providing a substrate with a positive photoresist thereon, forming a spot array valley in the positive photoresist using a spot pattern mask, forming an adhesive layer on the substrate and filling the spot array valley thereon, performing a full region exposure on the positive photoresist and removing the same, thereby leaving the adhesive layer as a spot array pattern on the substrate, and forming an immobilizing material cover the surface of the spot array pattern to obtain a 3-dimensional structure of the immobilizing material.