Patents Assigned to Codexis, Inc.
  • Transaminase reactions
    Patent number: 12247233
    Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
    Type: Grant
    Filed: May 23, 2022
    Date of Patent: March 11, 2025
    Assignee: Codexis, Inc.
    Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
  • Biocatalysts for the preparation of hydroxy substituted carbamates
    Patent number: 12241093
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: June 12, 2023
    Date of Patent: March 4, 2025
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
    Patent number: 12215368
    Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Grant
    Filed: April 23, 2020
    Date of Patent: February 4, 2025
    Assignee: Codexis, Inc.
    Inventors: Xiang Yi, Oscar Alvizo, Ravi David Garcia, David Entwistle, Charlene Ching, James Nicholas Riggins
  • Engineered glucose dehydrogenases and methods for the reductive amination of ketone and amine compounds
    Patent number: 12215359
    Abstract: The present application provides engineered glucose dehydrogenase polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Grant
    Filed: April 23, 2020
    Date of Patent: February 4, 2025
    Assignee: Codexis, Inc.
    Inventors: Xiang Yi, Oscar Alvizo, Ravi David Garcia, David Entwistle, Charlene Ching, James Nicholas Riggins, Nandhitha Subramanian
  • Engineered cyclic GMP-AMP synthase (cGAS) variant enzymes
    Patent number: 12180483
    Abstract: Cyclic GMP-AMP synthase (cGAS) enzymes have been engineered to produce polypeptides having increased cGAS activity in the cyclization of modified nucleoside triphosphates, including thiolated or fluorinated nucleoside triphosphates, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing cGAS enzymes, compositions comprising the cGAS enzymes, and methods of using the engineered cGAS enzymes are useful for the production of pharmaceutical compounds.
    Type: Grant
    Filed: April 1, 2022
    Date of Patent: December 31, 2024
    Assignee: Codexis, Inc.
    Inventors: Margie Tabuga Borra-Garske, Oscar Alvizo, Lillian Jasmine Miller, Aksiniya Lyubenova Petkova
  • Biocatalysts and methods for the synthesis of substituted lactams
    Patent number: 12163162
    Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
    Type: Grant
    Filed: February 3, 2023
    Date of Patent: December 10, 2024
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Paulina Elena Salim, Derek J. Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Q. Pham
  • Engineered DNA polymerase variants
    Patent number: 12129495
    Abstract: The present disclosure relates to engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The present disclosure also provides methods of using the engineered DNA polymerase polypeptides or compositions thereof for diagnostic and other purposes.
    Type: Grant
    Filed: October 14, 2022
    Date of Patent: October 29, 2024
    Assignee: Codexis, Inc.
    Inventors: Ericka Bermudez, David Elgart, Nikki D. Kruse, Mathew G. Miller, Vesna Mitchell, Jovana Nazor, Nandhitha Subramanian
  • T7 RNA polymerase variants
    Patent number: 12129494
    Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.
    Type: Grant
    Filed: December 21, 2021
    Date of Patent: October 29, 2024
    Assignee: Codexis, Inc.
    Inventors: Mathew G. Miller, Chinping Chng, Oscar Alvizo, Melissa Ann Mayo, James Nicholas Riggins, Xiang Yi, Jonathan S. Penfield, Gjalt W. Huisman, Jared Davis, Yasushi Saotome
  • Engineered pantothenate kinase variant enzymes
    Patent number: 12110513
    Abstract: The present invention provides engineered pantothenate kinase (PanK) enzymes, polypeptides having PanK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PanK enzymes are also provided. The present invention further provides compositions comprising the PanK enzymes and methods of using the engineered PanK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
    Type: Grant
    Filed: December 14, 2020
    Date of Patent: October 8, 2024
    Assignee: Codexis, Inc.
    Inventors: Margie Tabuga Borra-Garske, Oscar Alvizo, Lillian Jasmine Miller, Jesse B. Slaton, Aksiniya Lyubenova Petkova, Nandhitha Subramanian, Joshua Kolev, Anna Fryszkowska, Agustina Rodriguez-Granillo, Grant Murphy
  • Biocatalysts for ezetimibe synthesis
    Patent number: 12110512
    Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: March 17, 2021
    Date of Patent: October 8, 2024
    Assignee: Codexis, Inc.
    Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
  • Engineered purine nucleoside phosphorylase variant enzymes
    Patent number: 12110493
    Abstract: The present invention provides engineered purine nucleoside phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The present invention further provides compositions comprising the PNP enzymes and methods of using the engineered PNP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
    Type: Grant
    Filed: September 30, 2021
    Date of Patent: October 8, 2024
    Assignee: Codexis, Inc.
    Inventors: Scott J. Novick, Nikki Dellas, Vesna Mitchell, Da Duan, Jovana Nazor, Oscar Alvizo, Auric Anthony Sowell-Kantz, Jeffrey C. Moore, Mark A. Huffman, Agustina Rodriguez-Granillo, Deeptak Verma, Nicholas M. Marshall, Jay H. Russell, Keith A. Canada
  • PEROXIDASE ACTIVITY TOWARDS 10-ACETYL-3,7-DIHYDROXYPHENOXAZINE
    Publication number: 20240301367
    Abstract: The present invention provides engineered peroxidase enzymes, polypeptides having peroxidase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing peroxidase enzymes are also provided. The present invention further provides compositions comprising the peroxidase enzymes and methods of using the engineered peroxidase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.
    Type: Application
    Filed: May 6, 2024
    Publication date: September 12, 2024
    Applicant: Codexis, Inc.
    Inventors: Da Duan, Auric Anthony Sowell-Kantz, Aksiniya Lyubenova Petkova, Jovana Nazor, Nandhitha Subramanian, Oscar Alvizo
  • Penicillin-G acylases
    Patent number: 12084697
    Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.
    Type: Grant
    Filed: January 23, 2023
    Date of Patent: September 10, 2024
    Assignee: Codexis, Inc.
    Inventors: Oscar Alvizo, David Elgart, Robert Kevin Orr, James Nicholas Riggins, Anna Fryszkowska, Katrina W. Lexa, Xiang Yi, Da Duan, Courtney Dianne Moffett, Nikki Dellas, Vesna Mitchell
  • ENGINEERED GALACTOSE OXIDASE VARIANT ENZYMES
    Publication number: 20240287474
    Abstract: The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.
    Type: Application
    Filed: May 7, 2024
    Publication date: August 29, 2024
    Applicant: Codexis, Inc.
    Inventors: Christopher Micklitsch, Oscar Alvizo, Jovana Nazor, Harvinder Chagger Maniar, Mikayla Jianghongxia Krawczyk, Margie Tabuga Borra-Garske, Nandhitha Subramanian, Anna Fryszkowska, Nicholas M. Marshall, Agustina Rodriguez-Granillo, Deeptak Verma, Dewan Andrews
  • Engineered amylase variants
    Patent number: 12071642
    Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH<7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.
    Type: Grant
    Filed: September 6, 2023
    Date of Patent: August 27, 2024
    Assignees: Codexis, Inc., Société des Produits Nestlé S.A.
    Inventors: Chinping Chng, Nikki Dellas, Da Duan, Ravi David Garcia, Harvinder Chagger Maniar
  • Lov-D acyltransferase mediated acylation
    Patent number: 12060592
    Abstract: Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.
    Type: Grant
    Filed: November 4, 2020
    Date of Patent: August 13, 2024
    Assignee: Codexis, Inc.
    Inventors: Steven J. Collier, Ee Ling Teo, Joly Sukumaran, Robert J. Wilson, Junye Xu
  • ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS
    Publication number: 20240254457
    Abstract: The present application provides engineered polypeptides having imine or oxime reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Application
    Filed: April 17, 2024
    Publication date: August 1, 2024
    Applicant: Codexis, Inc.
    Inventors: Margie Borra-Garsek, Oscar Alvizo, Melissa Mayo, Stephan Jenne, Auric Anthoney Sowell-Kantz, Carmela Molinaro
  • Ketoreductase polypeptides for the production of azetidinone
    Patent number: 12049649
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: June 24, 2021
    Date of Patent: July 30, 2024
    Assignee: Codexis, Inc.
    Inventors: Onorato Campopiano, Emily Mundorff, Birthe Borup, Rama Voladri
  • Ketoreductase polypeptides for the preparation of phenylephrine
    Patent number: 12031159
    Abstract: The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.
    Type: Grant
    Filed: April 28, 2022
    Date of Patent: July 9, 2024
    Assignee: Codexis, Inc.
    Inventors: Oscar Alvizo, Steven J. Collier, Hans-Georg Joerg Hennemann, Seong Ho Oh, Wenjuan Zha
  • ENGINEERED 3-O-KINASE VARIANTS AND METHODS OF USE
    Publication number: 20240209408
    Abstract: The present invention provides engineered 3?O-kinase polypeptides useful for construction of materials used in template-independent polynucleotide synthesis, as well as compositions and methods of utilizing these engineered polypeptides. The present disclosure also describes one-pot methods for conversion of a natural or modified nucleoside to a nucleoside tetraphosphate or NQP.
    Type: Application
    Filed: October 12, 2023
    Publication date: June 27, 2024
    Applicant: Codexis, Inc.
    Inventors: David Entwistle, Stephanie Marie Forgel, Anders Matthew Knight, Mikayla Krawczyk, Philip Provencher, Amani Shoubber, Jonathan Vroom, David Watts, Leland Wong