Abstract: Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.
Type:
Grant
Filed:
November 30, 2007
Date of Patent:
October 4, 2011
Assignee:
Codexis Mayflower Holdings, LLC
Inventors:
Andreas Crameri, Willem P. C. Stemmer, Jeremy Minshull, Steven H. Bass, Mark Welch, Jon E. Ness, Claes Gustafsson, Phillip A. Patten
Abstract: Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.
Type:
Grant
Filed:
February 21, 2007
Date of Patent:
September 6, 2011
Assignee:
Codexis Mayflower Holdings, LLC
Inventors:
Steven H. Bass, Simon Christopher Davis, Phillip A. Patten, Matthew Tobin, Jeremy S. Minshull, Mark Welch, Claus Gustafsson, Brian Carr, Stephan J. Jenne, Sun Ai Raillard, Andreas Crameri, Willem P. C. Stemmer, Robin Emig, Pascal Longchamp, Stanley Goldman, Lorraine J. Giver, Joseph A. Affholter
Abstract: A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
Abstract: The present invention generally relates to methods of rapidly and efficiently searching biologically-related data space. More specifically, the invention includes methods of identifying bio-molecules with desired properties, or which are most suitable for acquiring such properties, from complex bio-molecule libraries or sets of such libraries. The invention also provides methods of modeling sequence-activity relationships. As many of the methods are computer-implemented, the invention additionally provides digital systems and software for performing these methods.
Type:
Application
Filed:
December 28, 2010
Publication date:
June 30, 2011
Applicant:
CODEXIS MAYFLOWER HOLDING, LLC
Inventors:
Claes Gustafsson, Sridhar Govindarajan, Robin A. Emig, Richard John Fox, Ajoy K. Roy, Jeremy S. Minshull, S. Christopher Davis, Anthony R. Cox, Phillip A. Patten, Linda A. Castle, Daniel L. Siehl, Rebecca Lynne Gorton, Teddy Chen
Abstract: In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators and libraries made by in silico shuffling methods are provided. One disclosed method involves identifying and producing a polypeptide. The method may involve (a) receiving data identifying sequences of two or more parental polypeptides or parental nucleic acids that encode the polypeptides; (b) selecting one or more cross-over sites on the sequences thereby defining one or more recombinant polypeptides or recombinant nucleic acids; (c) selecting at least one of the recombinant polypeptides or recombinant nucleic acids; (d) recombining one or more oligonucleotides, at least one of which has a sequence matching some or all of that of the recombinant polypeptides or recombinant nucleic acids selected in (c); (e) selecting at least some of the recombined oligonucleotides produced in (d); and (f) producing a polypeptide encoded by the selected nucleic acid.
Type:
Grant
Filed:
September 10, 2009
Date of Patent:
June 7, 2011
Assignee:
Codexis Mayflower Holdings LLC
Inventors:
Sergey A. Selifonov, Willem P. C. Stemmer, Claes Gustafsson, Matthew Tobin, Stephen del Cardayre, Phillip A. Patten, Jeremy Minshull, Lorraine J. Giver
Abstract: In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators are used to identify sets of oligonucleotides for in vitro recombination. In certain embodiments, the methods involve inputting amino acid sequence character strings into a digital system and back-translating the amino acid character strings into a plurality of nucleic acid character strings. The nucleic acid character strings are then processed to define and select at least one recombinant nucleic acid for use in identifying one or more oligonucleotides for in vitro recombination.
Type:
Grant
Filed:
October 31, 2007
Date of Patent:
March 8, 2011
Assignee:
Codexis Mayflower Holding, LLC
Inventors:
Sergey A. Selifonov, Willem P. C. Stemmer, Claes Gustafsson, Matthew Tobin, Stephen del Cardayre, Phillip A. Patten, Jeremy Minshull, Lorraine J. Giver
Abstract: Methods and systems for providing biological results in the form of systematically varied libraries of sequences or as data representing sequences or physical preparations of systematically varied libraries and/or selections from systematically varied libraries.
Type:
Grant
Filed:
August 20, 2002
Date of Patent:
January 18, 2011
Assignee:
Codexis Mayflower Holdings, LLC
Inventors:
Claes Gustafsson, Sridhar Govindarajan, Jeremy S. Minshull, Jon E. Ness, Robin A. Emig