Abstract: A method for preparing a topographically structured hydrogel microarray is described comprising the steps of a) providing one or more types of biomolecule(s) on top of micropillars of an array of micropillars, preferably by means of robotical spotting, b) providing a partially crosslinked hydrogel on a substrate, preferably attached to a substantially rigid and/or planar substrate, c) simultaneously soft-embossing a hydrogel microwell array and transferring the biomolecule(s) from the micropillars to the microwells by pressing the micropillars of the array of step a) onto the partially crosslinked layer of hydrogel of step b) until substantial completion of crosslinking and d) demolding the array of micropillars of step a) from the hydrogel microwell array of step c).
Type:
Grant
Filed:
September 12, 2012
Date of Patent:
August 13, 2019
Assignee:
Ecole Polytechnique Federale de Lausanna (EPFL)
Abstract: Peptides that specifically bind erythrocytes are described. These are provided as peptidic ligands having sequences that specifically bind, or as antibodies or fragments thereof that provide specific binding, to erythrocytes. The peptides may be prepared as molecular fusions with therapeutic agents, tolerizing antigens, or targeting peptides. Immunotolerance may be created by use of the fusions and choice of an antigen on a substance for which tolerance is desired.
Type:
Grant
Filed:
February 23, 2018
Date of Patent:
April 23, 2019
Assignee:
École Polytechnique Fédérale de Lausanna (EPFL)
Inventors:
Jeffrey A. Hubbell, Stephan Kontos, Karen Y. Dane
Abstract: Different types of energy storage systems are described, in particular hydro-pneumatic storage systems. In one, energy is stored by compressing gas in a chamber (44,45,54,55) with a liquid piston and released by gas expansion. A spray head or grid at the top of the chamber (44,45,54,55) supplies liquid as a shower through the gas being compressed or expanding in the cylinder (11,12) to maintain an isothermal condition. In another, energy is stored from an array of solar cells connected to an array of supercapacitors forming an auxiliary storage, and a main energy storage device such as a hydro-pneumatic storage system, for supply to an AC or DC network. The efficiency is improved by connecting the solar cells via the array of supercapacitors to the AC or DC network.
Type:
Grant
Filed:
May 9, 2007
Date of Patent:
February 19, 2013
Assignee:
Ecole Polytechnique Federale de Lausanna (EPFL)
Inventors:
Alfred Rufer, Daniel Favrat, Philippe Barrade, Sylvain Lemofouet