Abstract: Disclosed is a method for selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising the following steps: A) the fusion partners are brought into contact with each other and B) an electrical field of a strength sufficient to obtain fusion and highly focused on the fusion partners is applied. The fusion partners are independently selected from the group consisting of a single cell, a liposome, a proteoliposome, a synthetic vesicle, an egg cell, an enucleated egg cell, a sperm cell at any development stage and a plant protoplast.
Type:
Grant
Filed:
July 23, 2012
Date of Patent:
April 23, 2013
Assignee:
Collectricon AB
Inventors:
Peter Eriksson, Daniel T. Chiu, Alexander Moscho, Owe Orwar, Richard N. Zare