Abstract: The present invention relates to a device for collecting a sample of exfoliated cells from a colorectal mucosal surface of a human subject which comprises a colorectal insertion member (1) having a distal, insertion end (2), a proximal end (34) and a closable interior cavity (3), a flexible membrane having an outer, cell sampling surface (5) and an inner surface, wherein said membrane is sealingly attached to the distal, insertion end of the insertion member and held within the interior cavity, such that, in use, pressurization of the interior cavity to at least a first elevated pressure causes the membrane to emit from the distal end of the insertion member to make contact with the colorectal mucosal surface and pressurization of the interior cavity to a second reduced pressure causes the membrane to invert and return to the interior cavity of the insertion member. A kit comprising the device and methods of colorectal cell sampling using the device are also described.

Abstract: The present invention relates to a device for collecting a sample of exfoliated cells from a colorectal mucosal surface of a human subject which comprises a colorectal insertion member (1) having a distal, insertion end (2), a proximal end (34) and a closable interior cavity (3), a flexible membrane having an outer, cell sampling surface (5) and an inner surface, wherein said membrane is sealingly attached to the distal, insertion end of said insertion member and held within the interior cavity, such that, in use, pressurization of the interior cavity to at least a first elevated pressure causes the membrane to emit from the distal end of said insertion member to make contact with the colorectal mucosal surface and pressurization of the interior cavity to a second reduced pressure causes the membrane to invert and return to the interior cavity of said insertion member. A kit comprising said device and methods of colorectal cell sampling using said device are also described.

Abstract: A device for collection of exfoliated cells from the rectal mucosa comprises a hollow, cylindrical body having an inflatable and invertible flexible membrane attached to one end thereof. Applying positive pressure to inflate the membrane after the device is inserted, preferably through a rectal access tube, into the rectum causes exfoliated cells to be collected on the surface of the membrane. Before removal of the device, negative pressure is applied and the membrane, along with the collected sample of exfoliated cells, is deflated, inverted and withdrawn into the body of the device, thereby avoiding contact of the collected sample with body surfaces or the rectal access tube as the device is removed from the rectum.