Patents Assigned to CombiMatrix Corporation
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Publication number: 20100124767Abstract: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.Type: ApplicationFiled: June 22, 2009Publication date: May 20, 2010Applicant: COMBIMATRIX CORPORATIONInventor: Andrew V. Oleinikov
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Patent number: 7718579Abstract: A method for electrochemical removal of acid-labile protecting groups on an electrode microarray using an organic solution is disclosed. The solution comprises a hydrazine derivative and a salt in an organic solvent. The hydrazine derivative has at least one hydrazine group having at least one hydrogen. The hydrazine derivative provides acidic reagent when an electrode is active and isolates the acidic reagent to the area around the active electrode. The salt is an organic salt or ionic liquid having a concentration sufficient to provide electrochemical conductivity under an applied voltage. During the applied voltage, acidic reagent is generated, which removes acid-labile protecting groups thereby allowing continued addition of monomers to build a custom microarray of oligonucleotides, peptides, or other polymers.Type: GrantFiled: September 13, 2004Date of Patent: May 18, 2010Assignee: CombiMatrix CorporationInventor: Karl Maurer
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Patent number: 7563600Abstract: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.Type: GrantFiled: September 12, 2002Date of Patent: July 21, 2009Assignee: Combimatrix CorporationInventor: Andrew V. Oleinikov
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Patent number: 7541314Abstract: The present invention provides a microarray having base cleavable, sulfonyl-containing linkers and a process to make the microarray. Oligonucleotides of any sequence may be synthesized on the microarray having the cleavable linker. The oligonucleotides may be cleaved and recovered as a pool of oligonucleotides having a three prime phosphate moiety. Specifically, the microarray is an electrode containing microarray, and the oligonucleotides are electrochemically synthesized.Type: GrantFiled: February 24, 2006Date of Patent: June 2, 2009Assignee: CombiMatrix CorporationInventors: Dominic Suciu, Hetian Gao
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Patent number: 7507837Abstract: There is disclosed a process for performing an isolated Pd(II) mediated oxidation reaction electrochemically. The inventive process is performed on an electrode array device having a plurality of separately addressable electrodes. Preferably, the Pd(II) mediated oxidation is a Wacker reaction.Type: GrantFiled: December 7, 2005Date of Patent: March 24, 2009Assignee: CombiMatrix CorporationInventors: Kevin D. Moeller, Eden Tesfu, Karl Maurer
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Publication number: 20080039342Abstract: There is disclosed a process for performing an isolated Pd(0) catalyzed reaction electrochemically on an electrode array device. Specifically, there is disclosed a process for conducting an isolated Pd(0) catalyzed reaction on a plurality of electrodes, comprising providing an electrode array device having a matrix or coating material over metallic or conductive electrodes surfaces and a plurality of electrodes; providing a solution bathing the electrode array device, wherein the solution comprises a transition metal catalyst and a confining agent; and biasing one or a plurality of electrodes on the electrode array device with a voltage or current to regenerate the transition metal catalyst required for the isolated Pd(0) catalyzed reaction, whereby the confining agent limits diffusion of the transition metal catalyst to a volume surrounding each selected electrode surface.Type: ApplicationFiled: July 13, 2007Publication date: February 14, 2008Applicant: COMBIMATRIX CORPORATIONInventors: Jun Tian, Kevin Moeller, Sarah Wood, Karl Maurer
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Patent number: 7323320Abstract: There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.Type: GrantFiled: August 10, 2005Date of Patent: January 29, 2008Assignee: Combimatrix CorporationInventor: Andrew V. Oleinikov
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Publication number: 20070037169Abstract: The present invention provides a method for selective dehybridization by electrochemically-generated (ECG) reagent on an electrode microarray. The ECG reagent is generated by activation of selected electrodes. Activation alters pH in the vicinity of only the selected electrodes. In one embodiment, the increase or decrease in pH is sufficient to cause dehybridization of an oligonucleotide duplex at the selected electrodes. In another embodiment, the increase or decrease in pH is sufficient to prevent chemical dehybridization at the selected electrodes. The dehybridized single stranded target oligonucleotide may be recovered and amplified by PCR.Type: ApplicationFiled: August 9, 2005Publication date: February 15, 2007Applicant: COMBIMATRIX CORPORATIONInventors: John Cooper, Andrei Gindilis
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Patent number: 7075187Abstract: There is disclosed a coating material formulation for layering a plurality of electrodes to provide a substrate for the electrochemical synthesis of organic oligomers. Specifically, there is disclosed a coating layer of from about 0.5 to about 100 microns thick and is composed of a mixture of controlled porosity glass (CPG) particles having an average particle size of from about 0.25 to about 25 microns, and a thickening agent.Type: GrantFiled: November 9, 2001Date of Patent: July 11, 2006Assignee: CombiMatrix CorporationInventor: Karl Maurer
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Patent number: 6456942Abstract: The present invention provides methods for interfacing computer technology with biological and chemical processing and synthesis equipment. In preferred embodiments, the present invention features methods for the computer to interface with equipment useful for biological and chemical processing and synthesis in a remote manner.Type: GrantFiled: January 25, 2000Date of Patent: September 24, 2002Assignee: Combimatrix CorporationInventor: Brooke P. Anderson
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Patent number: 6444111Abstract: A solid phase synthesis method for the preparation of diverse sequences of separate polymers or nucleic acid sequences using electrochemical placement of monomers of nucleic acids at a specific location on a substrate containing at least one electrode that is preferably in contact with a buffering or scavenging solution to prevent chemical crosstalk between electrodes due to diffusion of electrochemically generated reagents.Type: GrantFiled: October 13, 1999Date of Patent: September 3, 2002Assignee: CombiMatrix CorporationInventor: Donald D. Montgomery
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Patent number: 6280595Abstract: A solid phase synthesis method for the preparation of diverse sequences of separate polymers or nucleic acid sequences using electrochemical placement of monomers or nucleic acids at a specific location on a substrate containing at least one electrode that is preferably in contact with a buffering or scavenging solution to prevent chemical crosstalk between electrodes due to diffusion of electrochemically generated reagents.Type: GrantFiled: September 10, 1999Date of Patent: August 28, 2001Assignee: CombiMatrix CorporationInventor: Donald D. Montgomery
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Patent number: 6093302Abstract: A solid phase synthesis method for the preparation of diverse sequences of separate polymers or nucleic acid sequences using electrochemical placement of monomers or nucleic acids at a specific location on a substrate containing at least one electrode that is preferably in contact with a buffering or scavenging solution to prevent chemical crosstalk between electrodes due to diffusion of electrochemically generated reagents.Type: GrantFiled: January 5, 1998Date of Patent: July 25, 2000Assignee: Combimatrix CorporationInventor: Donald D. Montgomery