Abstract: Test sensors, methods, and systems are described that include a first electrode pair having either two active electrodes or an inactive working electrode paired with an active counter electrode. These active electrodes are different than having an electron transfer mediator on an inactive electrode because in addition to the structural differences between an electrode directly in contact with the conductors of the test sensor verses a reagent coating, there are chemical and functional differences. The active electrodes are formed from an electrode core material including an element that loses or acquires electrons during the analysis and directly participates in the electrochemical reaction of the sample. As the active electrodes are insoluble in the sample during the analysis, an electrochemically stable potential is provided by the active electrodes that can reliably operate at higher operating potentials than conventional electron transfer mediator reagents coated on an inactive electrode.

Abstract: Test sensors, methods, and systems are described that include a first electrode pair having either two active electrodes or an inactive working electrode paired with an active counter electrode. These active electrodes are different than having an electron transfer mediator on an inactive electrode because in addition to the structural differences between an electrode directly in contact with the conductors of the test sensor verses a reagent coating, there are chemical and functional differences. The active electrodes are formed from an electrode core material including an element that loses or acquires electrons during the analysis and directly participates in the electrochemical reaction of the sample. As the active electrodes are insoluble in the sample during the analysis, an electrochemically stable potential is provided by the active electrodes that can reliably operate at higher operating potentials than conventional electron transfer mediator reagents coated on an inactive electrode.

Abstract: An electrochemical sensor includes a dielectric substrate and a conductive layer formed on a surface of the substrate. The conductive layer includes a working electrode, an electrode lead and a connecting arm connecting the working electrode to the electrode lead. A dielectric layer is positioned over the conductive layer. The dielectric layer has an aperture exposing the working electrode and a portion of the connecting arm. The working electrode, electrode lead and connecting arm may be formed by laser ablation technique to provide precise working electrode sensor parameters.

Abstract: An electrochemical sensor is provided comprised of a thin sheet or film having at least one through-hole provided therein. The thin sheet or film has first and second opposing surfaces, at least a portion of the opposing surfaces being coated with a conductive coating which extends into the at least one through-hole, each conductive coating extending partially into the at least one through-hole from each opposing surface whereby each conductive coating terminates within the at least one through-hole at a point spaced from the terminus of the opposing conductive coating which extends into the at least one through-hole, whereby the conductive coatings in the at least one through-hole are separated by a non-conductive interior surface of the at least one through-hole.

Abstract: An electrochemical sensor is provided comprised of a thin sheet or film having at least one through-hole provided therein. The thin sheet or film has first and second opposing surfaces, at least a portion of the opposing surfaces being coated with a conductive coating which extends into the at least one through-hole, each conductive coating extending partially into the at least one through-hole from each opposing surface whereby each conductive coating terminates within the at least one through-hole at a point spaced from the terminus of the opposing conductive coating which extends into the at least one through-hole, whereby the conductive coatings in the at least one through-hole are separated by a nonconductive interior surface of the at least one through-hole.