Abstract: Provided herein are compositions and devices for diagnosing and treating a viral infection in a subject and/or detecting a viral nucleic acid in a sample. In one example, a sample is treated in a first chamber and the sample is flowed into a second chamber, where any pathogenic nucleic acid is detected by oligonucleotides that are specific to the pathogen under test. Further, the oligonucleotides comprise a cleavage site for a restriction enzyme in the second chamber, which cleaves oligonucleotide-pathogenic nucleic acid hybrid resulting in the exposure of an enzyme that was being held by the oligonucleotide to its substrate, which generates a colorimetric and/or another visual readout.
Abstract: Provided herein are compositions, methods, assays, and devices for diagnosing and treating a viral infection in a subject and/or detecting a viral nucleic acid in a sample. In one example, a sample is treated in a first chamber and via a closing or twisting operation, the sample is flowed into a second chamber, where any pathogenic nucleic acid is detected by oligonucleotides that are specific to the pathogen under test. Further, the oligonucleotides comprise are cleavage site for a restriction enzyme in the second chamber, which cleaves oligonucleotide-pathogenic nucleic acid hybrid resulting in the exposure of an enzyme that was being held by the oligonucleotide to its substrate, which generates a colorimetric and/or another visual readout (e.g., foam formation). Further, the methods, device, or kits provided herein can be used to detect SARS-CoV-2 in a saliva sample from a human subject as a rapid in home diagnostic test for COVID-19.
Abstract: A threadless connector includes a connector body having a central bore therethrough and a retainer ring disposed in an end of the connector body. The retainer ring has a plurality of teeth segments inwardly angled to contact an end of a conduit inserted into the connector body.
Type:
Grant
Filed:
June 26, 2017
Date of Patent:
November 24, 2020
Assignee:
REPUBLIC CONDUIT, INC.
Inventors:
Sheridon Petersen, Barry Peters, Bruce Thompson
Abstract: A threadless connector includes a connector body having a central bore therethrough and a retainer ring disposed in an end of the connector body, the retainer ring comprising a plurality of teeth segments, wherein the plurality of teeth segments are configured to be inwardly angled to contact an end of a conduit inserted into the connector body.
Type:
Grant
Filed:
September 23, 2010
Date of Patent:
June 27, 2017
Assignee:
REPUBLIC CONDUIT, INC.
Inventors:
Sheridon Petersen, Barry Peters, Bruce Thompson